简介：Objective:TostudytheimmunologicalmechanismsofCondylomaacuminata(CA)throughinvestigatingTlymphocytesubsetlevelsandcytokineprofileintheperipheralbloodofpatientswithCondylomaAcuminata.Methods:Tricolorandbicolorimmunofluorescentstainingantibodyofcellsurfaceantigenandintracel-lularIL-2,IL-4,IL-12,IFN-γinCD4^+andCD8^+T-lymphocytesfrom20patientswithCAwereperformedandfollowedbyflowcytometry.Results:ThenumberofCD3^+T,CD4^+T-lymphocytescellsandCD4^+/CD8^+Tcellsratioweresignificantlydecreased(P<0.01)inpatientswithCAComparedtocontrols,andIL-2,IL-12,IFN-γproductioninCD4^+Tcellswasdecreased(P<0.01),IL-4andIFN-γproduc-tioninCD4^+Tcellswasnotsignificantlydifferent(P>0.05),whileIL-2andIL-12productioninCD8^+Tccellswasdecreased(P<0.01),whereasIFN-γandIL-4pro-ducinginCD4^+Tcellswereofnosignificantlydifference(P>0.05).Conclusions:TherewasanimbalanceofTlympho-cytesubsets,Th1/Th2cytokinesandTc1/Tc2intheperipheralbloodofCApatients,whichmayplayanimportantroleinthepathogenesisandprogressionofCA.

简介：Objective:ThepresentstudyaimedtoinvestigatecircularRNA(circRNA)expressioninuvealmelanoma(UM).Methods:First,weusedmicroarraytocomparetheexpressionprofilesofcircRNAinfiveUMsamplesandfivenormaluveatissues.Next,bioinformaticsanalyses,includinggeneontology(GO)analysisandpathwayanalysis,wereappliedtostudythesedifferentiallyexpressedcircRNAstopredictpathogenicpathwaysthatmaybeinvolved.Quantitativereal-timepolymerasechainreaction(qRT-PCR)in20UMsamplesand20normaluveasampleswasusedtoconfirmthecircRNAexpressionprofilesobtainedfromthemicroarraydata.Finally,weanalyzedtheinteractionbetweenvalidatedcircRNAsandtheirpotentialcancer-associatedmiRNAtargets.Results:Intotal,50,579circRNAs[foldchange(FC)≥2.0;P<0.05],including20,654up-regulatedand29,925down-regulatedcircRNAs,wereidentifiedasdifferentiallyexpressedbetweenUMtissuesandnormaluveatissues.WeusedqRT-PCRtoverifysevendysregulatedcircRNAsindicatedbythemicroarraydata,includinghsacirc0119873,hsacirc0128533,hsacirc0047924,hsacirc0103232,hsa-circRNA10628-6,hsacirc0032148andhsacirc0133460,whichmaybepromisingcandidatestostudyfuturemolecularmechanisms.Conclusions:Thisstudyexplored,forthefirsttime,theabnormalexpressionofcircRNAsinUManddescribedtheexpressionprofileofcircRNAs,providinganewpotentialtargetforthemechanismofUMandfuturetreatmentofUM.

简介：AbstractBackground:Recent studies have reported circular RNA (circRNA) expression profiles in various tissue types; however, circRNA expression profile in human epicardial adipose tissue (EAT) remains undefined. This work aimed to compare circRNA expression patterns in EAT between the heart failure (HF) and non-HF groups.Methods:RNA-sequencing was carried out to compare circRNA expression patterns in EAT specimens from coronary artery disease cases between the HF and non-HF groups. Quantitative real-time polymerase chain reaction was performed for validation. Comparisons of patient characteristics between the two groups were using t test, Mann-Whitney U test, and Chi-squared test.Results:A total of 141 circRNAs substantially different between the HF and non-HF groups (P < 0.05; fold change >2) were detected, including 56 up-regulated and 85 down-regulated. Among them, hsa_circ_0005565 stood out, for it had the highest fold change and was significantly increased in HF patients in quantitative real-time polymerase chain reaction validation. The top highly expressed EAT circRNAs corresponded to genes involved in cell proliferation and inflammatory response, including GSE1, RHOBTB3, HIPK3, UBXN7, PCMTD1, N4BP2L2, CFLAR, EPB41L2, FCHO2, FNDC3B, and SPECC1. The top enriched Gene Ontology term and Kyoto Encyclopedia of Genes and Genomes pathway were positive regulation of metabolic processes and insulin resistance, respectively.Conclusion:These data indicate EAT circRNAs may contribute to the pathogenesis of metabolic disorders causing HF.

简介：Domesticpig(Susscrofadomestica)isoneofthemostimportantmammalstohumans.Alternativesplicingisacellularmechanismineukaryotesthatgreatlyin-creasesthediversityofgeneproducts.Expressionsequencetags(ESTs)havebeenwidelyusedforgenediscovery,expressionprofileanalysis,andalternativesplicingdetection.Inthisstudy,atotalof712,905ESTsextractedfrom101differentnon-normalizedESTlibrariesofthedomesticpigwereanalyzed.TheseESTlibrariescoverthenervoussystem,digestivesystem,immunesystem,andmeatproductionrelatedtissuesfromembryo,newborn,andadultpigs,makingcontributionstotheanalysisofalternativesplicingvariantsaswellasexpressionprofilesinvariousstagesoftissues.AmodifiedapproachwasdesignedtoclusterandassemblelargeESTdatasets,aimingtodetectalternativesplicingtogetherwithESTabundanceofeachsplicingvariant.Mucheffortsweremadetoclassifyalternativesplicingintodifferenttypesandapplydifferentfilterstoeachtypetogetmorereliableresults.Finally,atotalof1,223geneswithaverage2.8splicingvariantsweredetectedamong16,540uniquegenes.Theoverviewofexpressionprofileswouldchangewhenwetakealternativesplicingintoaccount.

简介：

简介：

简介：AbstractBackground:Rectal cancer (RC) is a malignant tumor that seriously threatens human health. Long non-coding RNAs (lncRNAs) play a vital role in tumor regulation. Nevertheless, their exact expression features and functions remain obscure, and therefore was the aim of the current study.Methods:We utilized the Affymetrix human GeneChip to screen differentially expressed profiles of lncRNAs and mRNAs from the cancer tissues and matched paracancer tissues of 6 RC patients. Gene Ontology (GO) and pathway enrichment analyses identified crucial functions and pathways of the aberrantly expressed mRNAs. We used quantitative real-time polymerase chain reaction to verify the significant expression differences of 11 candidate lncRNAs between the cancer and paracancer tissues. LncRNA-mRNA coexpression networks were built by calculating the Pearson correlation value to identify significant correlation pairs. Online bioinformatics tools GEPIA2, ONCOMINE, and PROGgeneV2 were used to mine the expression and prognosis of three crucial mRNAs and six verified lncRNAs. Competing endogenous RNA networks were constructed by predicting microRNA response elements and calculating free energy.Results:We found 1658 differentially expressed lncRNAs (778 up-regulated and 880 down-regulated) and 1783 aberrantly expressed mRNAs (909 up-regulated and 874 down-regulated). GO and pathway enrichment analyses revealed the vital functions of the differentially expressed mRNAs, including cell proliferation, cell migration, angiogenesis, and cellular response to zinc ion. The canonical signaling pathways mainly included the interleukin-17, cell cycle, Wnt, and mineral absorption signaling pathways. Six lncRNAs including AC017002.2 (P= 0.039), cancer susceptibility 19 (CASC19) (P= 0.021), LINC00152 (P= 0.013), NONHSAT058834 (P= 0.007), NONHSAT007692 (P= 0.045), and ENST00000415991.1 (P= 0.045) showed significant differences in expression levels between the cancer tissue and paracancer tissue groups. AC017002.2, NONHSAT058834, NONHSAT007692, and ENST00000415991.1 have not yet been reported in RC. The crucial mRNAs myelocytomatosis viral oncogene (MYC), transforming growth factor beta induced (TGFBI), and solute carrier family 7 member 5 (SLC7A5) were selected. AC017002.2 and LINC00152 were positively correlated with MYC, TGFBI, and cytochrome P450 family 2 sub-family B member 6 (All r > 0.900, P < 0.050). NONHSAT058834 was positively associated with MYC (r = 0.930, P < 0.001), and CASC19 was positively correlated with SLC7A5 (r= 0.922, P < 0.001).Conclusion:This study offers convincing evidence of differentially expressed lncRNAs and mRNAs as potential biomarkers in RC.

简介：Objective:Ourgrouphaspreviouslyobservedthatinpatientswithsmall-celllungcancers(SCLCs),theexpressionofatumorantigen,gliomabigpotassium(gBK)ionchannel,ishigheratthetimeofdeaththanwhenthecancerisfirsttreatedbysurgicalresection.Thisstudyaimedtodeterminewhetherthisdichotomywascommoninotherpotentiallungtumorantigensbyexaminingthesamepatientsamplesusingourmoreextensiveprofileanalysisoftumor-antigenprecursorprotein(TAPP).WethentestedthehypothesisthattherapeuticinterventionmayinadvertentlycausethisincreasedgBKproduction.Methods:SCLCsamples(eightsurgicalresectionsandthreeautopsysamples)andthreecontrollungswereexaminedbyquantitativereal-timepolymerasechainreactionfor42potentialTAPPsthatrepresentpotentialT-cell-mediatedimmunologicaltargets.Results:Twenty-twoTAPPmRNAsdisplayedthesameprofileasgBK,i.e.,moremRNAswereexpressedatautopsythanintheirsurgicalcounterparts.B-cyclinandmousedoubleminute2,humanhomologofP53-bindingproteinwereelevatedinbothautopsyandsurgicalspecimensabovethenormal-lungcontrols.WhenHTB119cellswereincubatedwithdoxorubicin,gBKwasstronglyinduced,asconfirmedbyintracellularflowcytometrywithagBK-specificantibody.Conclusion:Ourfindingssuggestedthatmoreimmunologicaltargetsbecameavailableasthetumorrespondedtochemotherapyandproceededtowarditsterminalstages.

简介：Toexploretheeffectofenvironmentconditionsonimmuneactivityoffish,eightimmune-associatedgenesresponsibleforinnateimmunitywereselectedfromtheGenBank,i.e.Pgrn-a,Ifit2,P-hepcidin,Lect2,β2m,Irf1,Il25andHsp96,andthemRNAexpressionsoftheminthekidneyofculturedlargeyellowcroakerLarimichthyscroceaindifferentseaareasintheEastChinaSeawereexaminedwithqPCRtechniques.Inthecontrastsofimmune-associatedgeneexpressionbetweenareasandpopulations,significantdifferenceswerefound,expressionlevelsoftheseimmune-associatedgeneswerelowerintheclearwaterareathaninthepoorwaterquantityarea,andlowerinMaythaninOctober.MYwasmoresensitivetoenvironmentalfactorsthanDQ,whichwascoincidentwiththewaterqualityintheculturingareas.Differentialanalysesoftheexpressionlevelsoftheseimmune-associatedgenesshowedthatsignificantup-regulationcouldbetriggeredbypoorenvironmentalfactors.Theexpressionpatternsindicatedthattheexpressionlevelsofthesegenesweresensitivetoecologicalchanges,therebytheimmune-associatedgenes,especiallyPgrn-a,Ifit2,β2m,Il25andHsp96,mightserveasimmediateandsensitiveindicatorsofpopulationimmunologicvigorandecosystemhealth.Buttheexpressionofimmunity-associatedgenesatthelevelofgenetranscriptionishighlyinfluencedbymultiplefactors,andtheexactcausesorinfluencingfactorsoftheup-regulationordown-regulationofthesegenesstillneedfurtherthoroughinvestigation.

简介：

简介：

简介：

简介：Dr.YanMaochao(1963-),male,anativeofJingshanCountyofHubeiProvince,co-founderofInternationalSocietyfortheAdvancementofEmergyResearch(ISAER),memberofChinaSocietyofEcologicalEconomics

简介：布莱恩·斯戚尼通用汽车前不久任命布莱恩·斯威尼（BrianSweeney）为旗下别克和GMC品牌负责人。在此之前，布莱恩·斯威尼已经为通用服务了20年，他在通用汽车担任别克和GMC销售经理等职务，主要负责经销商的销售工作。

简介：

简介：

简介：直译：像马一样吃东西。

简介：Tobesickasadog.直译：病得像狗一样。意译：病得很严重。

简介：直译：裤子里有蚂蚁。意译：形容焦躁不安的心情。

简介：