简介：LipasewasimmobilizedbycovalentbindingoncrosslinkedallyldextranusingSESAascouplingagent.Itisshownthatthisimmobilizationapproachisanefficientoneforlipase.Theactivityoftheimmobilizedlipasecanreachto300-450U/g(dryweight).Itexhibitsgoodtemperaturestability,canretain88%activityafterbeingincubatedat70℃for2h.Specialeffectswillbeexpectedfromourimmobilizedlipaseinitsapplicationsinorganicmediaduetothenatureofthesupport.

简介：ImmobilizationusinglittlesplintisanoriginalinnovationofChinesepeopleforthefracturefixation,whichissimpletouseandclinicallyeffective.ItwasfoundthatChineseimmobilizationusinglittlesplintcanmakethenon-invasive,uncovering,andtroublefreehealingofbonefractureviaharmoniousunityofthestructurestabilityandtheforcebalance,ofthemotionstabilityandthestressadaptability,oftheconstantanddiscontinuousphysiologicalstress.ThebiomechanicaleffectofChineseimmobilizationusinglittlesplint,includingentirety,dynamic,andfunctionalfixity,istherootcauseofitsinheritanceandtheuseuptonow,andalsoisadirectionoftoday’sfracturefixationtowardspersonalization,individualityandentirety.

简介：自从工业革命的开始，用pyrometallurgical过程的金属精制植物产生了铅(Pb)和镉(Cd)的巨大的排出物。作为如此的污染物质的核心目标，很多土壤现在在普遍区域上被污染，形成对世界范围的公共健康的大威胁。不同于器官的污染物质，Pb和Cd不经历化学或微生物引起的故障并且在他们的版本以后为更长的持续时间在地点保持可能。固定是一在原处使用划算的土壤修正案在污染土壤减少Pb和Cd可获得性的补习技术。在土壤环境的Pb和Cd污染与来源丰富，种形成和联系健康的焦点被考察用各种各样的土壤修正案的风险，和固定选择。通常适用并且为Pb和Cd固定的新兴的划算的土壤修正案包括磷酸盐混合物，liming，动物粪肥，biosolids，金属氧化物，和biochar。这些使不能调动的代理人能把金属污染物质或残余的转移归结为食物网(植物举起并且沥滤到表面下的水)并且他们在重金属固定的长期的可持续性需要另外的评价。

简介：Thisstudyisconcernedwithchitosan-polyacrylicacidcomplexasacarriertoimmobilizeglucoseoxidase(GOD)andcellulase.TheoptimumemperatureoftheimmobilizedGOD(IG)wasdeterminedtobe60℃whichishigherthanthatofthenativeGODabout40℃.Theoptimumtemperatureoftheimmobilizedcellulase(IC)wasdeterminedtobeabout30℃higherthanthatofnativecellulase.BothoftheoptimumpHofIGandICshiftedonepHunittoacid.ImmobilizedenzymemaybeusedinmorewidepHrange.Theirstoragelifearemuchlongercomparedwiththeirnativestates.Bothofthemcanbereusedatleast12times.

简介：Macroporousmethylacrylate-divinylbenzenecopolymersweresynthesizedandmodifiedbypolyethylenepolyamine.TheaminoacylasefromAspergillusoryzaewasadsordedonthesemodifiedcopolymers.Theroleofcertainfactorssuchasdegreeofcross-linking,amountofporegenicsolvent,modifyingagentandporestructures,intheactivityofimmobilizedaminoacylasewasstudied.EffectsofadsorptiontimeandamountofenzymeusedontheactivityofaminocylaseimmobilizedonMMD-12-13werealsoinvestigated.ThecolumnofaminoacylaseimmobilizedonMMD-12-13waspreparedandN-acyl-DL-methioninewasresolvedcontinuouslyforamonth,theremainingactivitywasstillover90%.

简介：ＤＥＶＥＬＯＰＭＥＮＴＯＦＡＶＥＲＳＡＴＩＬＥＳＥＮＳＩＮＧＭＥＭＢＲＡＮＥＦＯＲＩＭＭＵＢＩＬＩＺＡＴＩＯＮＯＦＰＲＯＴＥＩＮＡＮＤＡＮＥＷＩＭＭＯＢＩＬＩＺＡＴＩＯＮＭＥＴＨＯＤＹ．Ｋ．Ｚｈｏｕ，Ｊ．Ｗ．Ｙｕａｎ，Ｓ．Ｑ．Ｘｕ，Ｂ．Ｈ．Ｓｈｕ（Ｄ...

简介：Aseriesofpoly(methylacrylate)copolymersofdifferentporestructuresweresynthesizedandfunctionalizedbypolyethylenepolyamine.Thelipasefromporcinepancreaswasadsorbedonthesepolymercarriers.Itwasfoundthattheproestructureandfunctionalgroupwerebasicfactorswhichaffectedtheactivityofimmobilizedlipase,TheoptimalconditionsforadsorbinglipasewerestudiedandtheeffectsofpH,ionicstrengthandtemperatureontheimmobilizedlipasewerecomparedwiththoseonthedissolvedlipase.

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简介：Thepurposeofthisresearchistoinvestigatethepotentialofpre-treatmentwithphosphoricacid(PA)andmonobasiccalciumphosphate(MCP)forthestabilizationofheavymetalsinsludgeanditsbottomash.ThetannerysludgesampleswerecollectedinWenzhou,Chinaandheavilycontaminatedwithheavymetals,suchasPb,Crandsoon.Thesampleswerepre-treatedwithPAorMCP.ThenXRDandTCLPtestswereadoptedastheevaluatingmethodstocharacterizethePb,Cr,Cu,ZnandCdimmobilizationinthepre-treatedsludgeanditsbottomash.TheresultsshowedthatthistreatmenteffectivelyimmobilizedPbandCdinthesludge,lightlystabilizedthemetalCu,andadversely,enhancedtheleachabilityofZn.Afterincinerationat900℃,PbandCrinthebottomashofpre-treatedsludgeweresignificantlystabilizedduetotheformationoftheirhighlythermostablephosphatesandpyromorphite-likemineralsduringtheincinerationprocess.However,anincreaseofCuandZnsolubilitywasobservedwhichmightbeattributabletotheacidificationofsludgeduetotheadditionofPAorMCP.

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简介：Seriesofpoly(methylacrylate)copolymersofdifferentporestruc-turesweresynthesizedanddirectlyaminatedbypolyethylenepolyamine.Thelipacefromporcinepancreaswasadsorbedonthesecopolymers.Theactivityofimmobilizedlipasewasinfiuencedbydifferentpolyethylenepolyamines,thedegreeofcross-linkingandtherelativerolumeofpore-generatingsolvent.

简介：ChiralRu-BsDPEN,(1R,2R)-N-p-benzenesulfonyl-1,2-diphenylethylenediamine,catalysthasbeenimmobilizedonamesoporousmolecularsieveofMCM-41typesuccessfully.Ahybridmesoporousmolecularsievewassynthesizedusingaprecursorbearingbenzenegroup,whichinorganosilicaweresulfonylatedandreactedwith(1R,2R)-l,2-diphenylethylenediamineand[RuC1E(p-cymene)]2successivelytoaffordimmobilizedcatalyst.TheBrunauer-Emmett-Teller(BET)surfaceareaandBarrett-Joyner-Halenda(BJH)poresizedecreasedafterimmobilizationofcatalystontothemesoporousmaterial.Enantioselectivetransferhydrogenationofketonescatalyzedbyimmobilizedcatalystshowedthehighestyieldof22.36%ande.e.valueof31.47%byusingacetophenoneassubstratewhenreactiontimewas48and16hrespectively.

简介：MethacrylicacidfirstwasneutralizedwithanaqueoussolutionofNaOHtopH=6.0～7.0,vinylenecarbonate(VCA)wasaddedtothesolution,thenmonomerswerecopolymerizedinparaffinoilbymeansofreverse-phasesuspensionpolymerizationandhydrophiliccopolymericsupportswereprepared.ThepropertiesofthesupportsweredeterminedusingtrypsinandresultsshowthattheamountofenzymescoupledtothesupportsandthespecificactivityofimmobilizedtrypsinarerelatedtothecontentofVCAstructureunits,reactiontimeandconcentrationofenzymesolution,etc.

简介：Immobilizingbiocomponentsonsolidsurfacesisacriticalstepinthedevelopmentofnewdevicesforfuturebiological,medical,andelectronicapplications.Therefore,numerousintegratedfilmswererecentlydevelopedbyimmobilizingdifferentproteinsorenzymesonelectrodesurfaces.Inthiswork,hemeproteinsweresafelyimmobilizedontomacroporousnickel-basedelectrodeswhilemaintainingtheirfunctionality.Suchmodifiedelectrodesshowedinterestingpseudo-capacitivebehavior.Amonghemeproteins,hemoglobin(Hb)filmhasahigherelectrochemicalperformanceandgreatercharge/dischargecyclingstabilitythanmyoglobin(Mb)andcytochromeC(CytC).Thehemegroupinanalkalinemediumcouldinducetheformationofsuperoxidesontheelectrodesurface.Thesecapacitivefeaturesofhemeprotein-NielectrodewererelatedtostrongbindingsitesbetweenhemeproteinsandporousNielectrode,theaccumulationofsuperoxideorradicalsontheNisurface,andfacileelectrontransferandelectrolytediffusionthroughthethree-dimensionalmacroporousnetwork.Thus,thesenewprotein-basedsupercapacitorshavepotentialuseinfree-standingplatformtechnologyforthedevelopmentofimplantableenergy-storagedevices.

简介：HeparinwascovalentlyimmobilizedontopolyurethanesurfaceviaaPEGspacerbyamicrowave-assistedapproachtoimprovebloodcompatibility.Firstly,amino-terminatedpoly(ethyleneglycol)(APEG)wasrapidlygraftedontoPUsurfacewithin20minbyatwo-stepmethodinvolvingmicrowave-assistedMDI-functionalizationandsubsequentmicrowave-assistedAPEGcoupling.Then,heparinwascovalentlyimmobilizedthroughanamidelinkagebythedirectcouplingofthecarboxylicacidofheparinwiththeaminogroupofAPEGonPUsurfaceusingcarbodiimidecouplingreaction.ThesurfacestructureandpropertieswerecharacterizedbyX-rayphotoelectronspectroscopy(XPS),atomicforcemicroscopy(AFM)andwatercontactanglemeasurements.Theresultsrevealedthatheparin-immobilizedPUsurfacehadslightlyincreasedroughnessandsignificantlyimprovedhydrophilicityincomparisontotheoriginalPUsurface.Theanticoagulantactivityoffilmswasevaluatedbywholebloodclottingtime(CT)andprothrombintime(PT).Complementactivationwasassessedbydetectingcomplementfragment3aconcentrationsofserumexposedtothefilms.Theresultsrevealedthatthemicrowave-assistedheparin-immobilizedPUfilmshadexcellentantithrombogenicityandsuppressedcomplementactivation,indicatingimprovedbloodcompatibility.

简介：UV-inducedgraftpolymerizationofacrylicacid(AA)onpoly(etheretherketone)(PEEK)filmswascarriedouttointroduce―COOHforthesubsequentimmobilizationofbovineserumalbumin(BSA).BSAwasintroducedonPEEKsurfacebasedonthecondensationreactionbetween―NH2and―COOH.Themodifiedsurface(PEEK-BSA)wascharacterizedbyenergy-dispersespectrometry(EDS),X-rayphotoelectronspectroscopy(XPS),watercontactanglemeasurementandUVspectrumanalysis.Thecontactanglewasfoundtodecreasefrom104°forthevirginPEEKfilmsto63°fortheBSA-immobilizedPEEKfilms,demonstratingasignificantimprovementofsurfacehydrophilicity.Moreover,theappearanceofnitrogenonPEEKfilmconfirmedbyXPSandEDSindicatestheimmobilizationofBSAonPEEKsurface.

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