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简介:内容摘要:新时期院校的体能训练方法在不断探索创新,为了更好的提高训练质量,需要不断做到合理化,理性化、科学化。侧重培养学员的主动训练意识,使学员养成良好的训练习惯,提高自我锻炼的能力。“3+1”学员为了更好的完成职责任务必须练就过硬的军事技能,而体能素质是一切军事技能的基础,如何提高学员的体能素质,把科学方法、生理运动和卫生健康等多门学科运用于训练中,结合人体运动学,最大限度地减少训练对人体造成的伤害,达到提高军事训练质量的目的。是摆在我们面前深刻思考和研究的问题。
简介:摘要目的将聚己内酯(PCL)薄膜与1,1′-双十八烷基-3,3,3′,3′-四甲基吲哚碳菁高氯酸盐(DiI)标记的骨髓间充质干细胞(BMSCs)共培养,制备细胞补片。方法取1只清洁级SD大鼠作为实验对象,通过分离培养获得大鼠BMSCs,并进行流式细胞仪鉴定表面标志物。BMSCs培养至3代后,使用DiI染料对BMSCs标记,将DiI标记的BMSCs与PCL制成的薄膜共培养,24 h后在荧光显微镜下观察细胞生长情况,并固定进行电镜扫描,在培养的1、4、7、10 d分别用细胞计数试剂盒(CCK-8)检测细胞增殖。多组间比较采用单因素方差分析,两两比较采用LSD分析。结果BMSCs流式细胞术鉴定:CD90、CD44H强阳性,CD11b/c、CD45阴性。荧光显微镜下,可见DiI标记的BMSCs发红光,呈梭形或多边形。扫描电镜下,PCL薄膜与DiI标记的BMSCs共培养形成的细胞补片,其表面细胞数量多,细胞状态正常。CCK-8测定结果显示第1天吸光度(A)值为0.330±0.025;第4天A值为0.620±0.012,第7天A值为1.033±0.144,第10天A值为1.223±0.133。各时间点A值之间差异有统计学意义(F=66.441,P<0.05)。结论PCL薄膜可以作为一种DiI标记示踪的支架材料,用于干细胞治疗。
简介:摘要6月龄患儿以腹泻、呕吐起病,临床表现为排稀水样便并次数增多,营养不良,双肾多发结石,胃肠镜示慢性浅表性胃窦炎、慢性结肠炎,十二指肠降段黏膜病理示固有层多量淋巴细胞、浆细胞浸润伴绒毛萎缩。全外显子测序发现STAT3基因c.1516G>A(p.E506K)杂合错义突变,为自发变异。该突变未曾见报道,可能是儿童慢性腹泻新发致病突变。该患儿改无乳糖深度水解配方喂养及对症支持治疗后排便正常,体重逐渐增加,双肾结石变小。
简介:摘要:英语语言的学习是语言知识、语言技能、情感策略、学习策略、文化意识的统一。因此语言结构的整体性、完整性与发展性是紧密相联、互相依存的整体。本文依据人教版初中英语预备单元的教学内容,对预备单元的教学内容进一步分析和重建,并针对预备单元教学中存在的问题提出了一些自己的建议。
简介:AbstractBackground:Microglia plays an indispensable role in the pathological process of sleep deprivation (SD). Here, the potential role of microglial CX3C-chemokine receptor 1 (CX3CR1) in modulating the cognition decline during SD was evaluated in terms of microglial neuroinflammation and synaptic pruning. In this study, we aimed to investigat whether the interference in the microglial function by the CX3CR1 knockout affects the CNS’s response to SD.Methods:Middle-aged wild-type (WT) C57BL/6 and CX3CR1-/- mice were either subjected to SD or allowed normal sleep (S) for 8 h to mimic the pathophysiological changes of middle-aged people after staying up all night. After which, behavioral and histological tests were used to explore their different changes.Results:CX3CR1 deficiency prevented SD-induced cognitive impairments, unlike WT groups. Compared with the CX3CR1-/- S group, the CX3CR1-/- SD mice reported a markedly decreased microglia and cellular oncogene fos density in the dentate gyrus (DG), decreased expression of pro-inflammatory cytokines, and decreased microglial phagocytosis-related factors, whereas increased levels of anti-inflammatory cytokines in the hippocampus and a significant increase in the density of spines of the DG were also noted.Conclusions:These findings suggest that CX3CR1 deficiency leads to different cerebral behaviors and responses to SD. The inflammation-attenuating activity and the related modification of synaptic pruning are possible mechanism candidates, which indicate CX3CR1 as a candidate therapeutic target for the prevention of the sleep loss-induced cognitive impairments.
简介:【摘 要】龙华中路住宅项目是本公司应用EPC总承包模式的住宅工程,项目整合优势资源,精心策划,对项目的设计、质量、进度、造价等各方面整体去把控,明确各方职责,面对EPC承包模式在界面交错,参建单位众多等问题和困难,有效预判并规避风险,在最短的工期条件下,成功完成了项目建设任务并按时交付。