简介：AbstractSingle-domain antibodies have the characteristics of small molecular weight, strong tissue penetration, and high affinity, and are widely used to construct molecular probes for disease diagnosis and treatment. This article reviews molecular imaging studies including positron emission tomography (PET), single-photon emission computed tomography/computed tomography (CT), PET/CT, and fluorescent imaging of molecular probes composed of single-domain antibodies against eight esophageal squamous cell carcinoma biological targets. These 8 targets are highly expressed on the membrane of esophageal squamous cell carcinoma cells and include epidermal growth factor receptor, human epidermal growth factor receptor 2, human epidermal growth factor receptor 3, hepatocyte growth factor receptor, vascular endothelial growth factor receptor 2, chemokine receptor 4, chemokine receptor 7, and carcinoembryonic antigen. The current problems and solutions are also discussed to provide a reference for future design of molecular imaging probes targeting esophageal squamous cell carcinoma.

简介：InordertoimprovethefunctionalaffinityofthehumanizedVHsingledomainantibodyagainsthumanlungcancer,thegenescodingthehomogenousdimersdihu3D3V_Handtetramerstehu3D3V_HwereconstructedbyfusingtheSVS-Cysshortpeptideandp53tetramerizationstructuraldomaingenetohu3D3V_HgeneviarecombinantPCRtechnique,respectively.Then,thedihu3D3V_Handtehu3D3V_HgeneswereclonedtotheprokaryoticexpressionvectorpET-22b(+)andexpressedinE.coliBL21(DE3).TheproteinsexpressedwerepurifiedthroughNi~(2+)-affinitychromatographiccolumn.Meanwhile,thehu3D3V_H,dihu3D3V_Handtehu3D3V_HproteinswerelabeledwithFITC,andtheirreactivitywithan-tigenandspecificitywereanalyzedbyimmunofluorescenceassay.Astotheirfunctionalaffinities,itwasanalyzedandcomparedbyflowcytometry.Theresultsindicatedthatthesetwogeneswereexpressedasmonomersandmainlyasinclusionbodies.Afterpurificationandrenaturation,therewereabout50%ofdimersand70%oftetramerremainingintheproteinsolution.Inaddition,thedihu3D3V_Handte-hu3D3V_Hproteinsstillremainedthereactivitywithantigenandspecificityofhu3D3V_Hprotein,andtheirfunctionalaffinitieswereincreasedabout60%or100%respectively,comparedwiththoseofhu3D3V_Hprotein.Itisevidentthatthefunctionalaffinityofhu3D3V_Hproteincanbegreatlyimprovedbyincreasingitsbindingvalency.

简介：在这份报纸，我们由电气化学的蚀刻生产多孔的硅(希腊语的第二十三个字母)，并且为重链的抗体(VHH)的重链的可变领域的察觉评估没有标签的多孔的硅生物传感器的性能是第一次。母鸡鸡蛋白人溶解酵素(HEWL)和VHH的绑定在生物传感器的思考光谱引起红移动。红移动与在从14g的范围的VHH集中成正比??踐?迱

简介：Topreparemonoclonalantibodyspecifictoepidermalgrowthfactorreceptor(EGFR)intracellulardomain,itsgenewasamplifiedfromtotalRNAofA431cellbyRT-PCR.ThenthegenewasclonedintoprokaryoticvectorpET30a(+).TherecombinantplasmidwastransformedintoE.ColiBL21(DE3)strainforproteinexpression.RecombinantproteinwasinducedwithIPTGandpurifiedusingNi2+-NTAagarose.Thentheanti-EGFRmonoclonalantibody(nAb)waspreparedwithclassicalhybridomatechnique.Positivecloneswereselectedusingindirectenzyme-linkedinmunoabsorbentassay(ELISA).Totally4hybridomacloneswereobtainedandthesemAbswereIgG1(3clones)andIgG2a(1clone),respectively.Theirlightchainswereallkappachains.WesternblottinganalysisandconfocalimmunofluorescenceassaysdemonstratedthatmAbscouldspecificallyrecognizeEGFRexpressingonA431carcinomacellline.ThemAbswillbeusefulinthestudyofEGFR-mediatedsignaltransduction.

简介：TofindoptimalconditionsforexpressingthesolubleformofsFv-2F3andtostudythepurificationandpropertyofitsderivativeSe-sFv-2F3,thepreferredexpressionconditionswereinvestigatedbymeansoforthogonaldesign.Thesecultureconditionsincludedincubationtemperature,inducerconcentration,inductiontimeandcellconcentration.TheevaluationofexpressionwasaccomplishedbytheanalysisofwholecelllysatesandtheyieldofsolublesFv-2F3wascalculatedaccordingtotheanalysisofProfinder(FTI-500,Pharmacia).Thepurificationprocedurewascarriedoutviaatwo-steppurificationprocedureconsistingofion-exchangechromatography,followedbyimmobilizedmetalaffinitychromatography(IMAC).TheantioxidantefficacyofSe-sFv-2F3wasdemonstratedbythedeterminationofthecontentofthemainproductoflipidperoxidation,MDA,theviabilityofcellsandtheactivityofLDH.WeobtainedthepreferredcultureconditionstogrowtheengineeredbacteriaandtheprocedureforpreparingsolublesFv-2F3andconfirmedtheantioxidantefficacyofSe-sFv-2F3.

简介：IntroductionThereisnowanewwaytotreathypercholesterolemia,usingamonoclonalantibody(Evolocumab)thatbindstoandinhibitsPCSKA9.MostphysiciansknowthetermPCSK9buthavenoideawhatPCSK9standsfor(includingme).MypurposeforwritingthiseditorialistoeducatemyselfonwhatPCSK9isandwhatitdoes.MyhopeisthatIcanperhapseducatethosereadingthisdocumentaswell.

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简介：Inordertoacknowledgethemulti-infectiondataofSTDpatientsandimprovetheprophylaxisandtherapyforSTDs，297patientsand30healthypeoplewereexaminedusingtheHSV-2plasmaantibody．TheresultsshowedthatallkindsofSTDpatientswereinfectedbyHSV-2indifierentratios．thehighestratiooccurringinsyphilispatients．

简介：1PURPOSOFTHESTUDYThearticle99intheSpecificRulesforEnforcingtheFrontierHealthandQuarantineLawofthePeople’sRepublicofChinahasindicatedthatthehealthandquarantineorganizationshallpreventanyforeignersufferingfromvenerealdiseasesfromenteringtheterritory.Becausetheroutineconfirmatorytestforsyphilisfluorescenttreponemalantibodyab-

简介：Objective:Toevaluatetheclinicalutilityoftestingserumanti-treponemapallidumIgMantibodyinthediagnosisofsyphilispatients.Methods:Seventy-twocasesofsyphilisweretestedforspecificIgMantibodywithELISA,andtheresultswerecomparedwithRPRandTPPA.Results:ThesensitivityofIgMantibodywas73.3%(11/15)inprimarysyphilis,88.9%(16/18)insec-ondarysyphilis,andtherewasnosignificantdiffer-encebetweenthesevalues(x^2=1.6363,P>0.10).ThesensitivityofIgMantibodyindiagnosinglatentsyphi-liswasonly26.1%(6/23),muchlowerthanthedetec-tionrateinsymptomaticearlvsvDhilis(x^2=17.6189.P<0.005).RPRandTPPAwereboth100%sensitiveinlatentandearlysymptomaticsyphilis.Twowereposi,fiveforIgMinthe16caseswhohadreceivedregulartreatments2to24monthsbeforeenrolled.Conclusions:SpecificIgMantibodydetectiondoeesnotappearsuperiortoRPRandTPPAindiagnosingprimarysyphilis.ThediagnosisoflatentsyphilisshouldmainlyrelyonRPRandTPPA,sincetherearelowtitersofIgMantibodyatthatstage.IgMantibodytestingaloneshouldnotberecommendedformonitor-ingsyphilisdevelopmentortreatmentefficacy.Fur-therstudiesshouldbeconcerned.

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简介：在时间域的领域分解和匹配的方法为模仿在波浪进展的轮船的运动被构画出。流动域被想象的控制表面分解成内部、外部的域，并且当短暂格林函数方法在外部域被使用时，Rankine来源方法被用于内部域。二个起始的边界价值问题在控制表面上被匹配。相应数字代码被开发，并且为系列60轮船和S175货柜船在头海进展的增加的群众，波浪激动力量和轮船运动，被介绍并且验证。当数字结果与试验性的数据和另外的引用相比时，一个好协议被获得了。它证明因为面板discretization，现在的方法在数字计算期间在内部领域仅仅是更有效的，并且好数字稳定性被证明与闪光关于轮船避免分叉问题。

简介：ＳＴＵＤＹＯＮＭＥＴＡＳＴＡＳＩＳＡＳＳＯＣＩＡＴＥＤＧＥＮＥＳＣＲＥＥＮＥＤＢＹＭＯＮＯＣＬＯＮＡＬＡＮＴＩＢＯＤＹＨＩＬＱｉＴｅｎｇｐｉｎｇ齐藤平；Ｚｈａｎｇｐｅｉｊｉ张沛基；ＷｅｉＳｈｕｇｕａｎｇ魏曙光；ＣｈｅｎＤｏｎｇ陈东；ＬｉＲｅｎ李仁；Ｗ...

简介：SeveralcubatureformulasonthecubicdomainsarederivedusingthediscreteFourieranalysisassociatedwithlatticetiling,asdevelopedin[10].ThemainresultsconsistofanewderivationoftheGaussiantypecubaturefortheproductChebyshevweightfunctionsandassociatedinterpolationpolynomialson[-1,1]~2,aswellasnewresultson[-1,1]~3.Inparticular,compactformulasforthefundamentalinterpolationpolynomialsarederived,basedonn~3/4+(?)(n~2)nodesofacubatureformulaon[-1,1]~3.

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简介：Thissurveyreviewstherecentdevelopmentofgradientdomainmeshdeformationmethod.Differenttootherdeformationmethods,thegradientdomaindeformationmethodisasurface-based,variationaloptimizationmethod.Itdirectlyencodesthegeometricdetailsindifferentialcoordinates,whicharealsocalledLaplaciancoordinatesinliterature.BypreservingtheLaplaciancoordinates,themeshdetailscanbewellpreservedduringdeformation.DuetothelocalityoftheLaplaciancoordinates,thevariationaloptimizationproblemcanbecastedintoasparselinearsystem.Fastsparselinearsolvercanbeadoptedtogeneratedeformationresultinteractively,oreveninreal-time.Thenonlinearnatureofgradientdomainmeshdeformationleadstothedevelopmentoftwocategoriesofdeformationmethods:linearizationmethodsandnonlinearoptimizationmethods.Basically,thelinearizationmethodsonlyneedtosolvethelinearleast-squaressystemonce.Theyarefast,easytounderstandandcontrol,whilethedeformationresultmightbesuboptimal.Nonlinearoptimizationmethodscanreachoptimalsolutionofdeformationenergyfunctionbyiterativeupdating.Sincethecomputationofnonlinearmethodsisexpensive,reduceddeformablemodelsshouldbeadoptedtoachieveinteractiveperformance.Thenonlinearoptimizationmethodsavoidtheuserburdentoinputtransformationatdeformationhandles,andtheycanbeextendedtoincorporatevariousnonlinearconstraints,likevolumeconstraint,skeletonconstraint,andsoon.Wereviewrepresentativemethodsandrelatedapproachesofeachcategorycomparativelyandhopetohelptheuserunderstandthemotivationbehindthealgorithms.Finally,wediscusstherelationbetweenphysicalsimulationandgradientdomainmeshdeformationtorevealwhyitcanachievephysicallyplausibledeformationresult.