简介：摘要：分类推进人才评价机制改革，建立科学的人才分类评价机制，调动人才创新创业积极性，对于国有企业转型升级、全面深化改革成功具有十分重要的战略意义，所以我们必须加快分层分类人才评价机制的建立健全。以“德”、“能”、“勤”、“绩”、“廉”五个要素，研究建立以企业中基层员工为对象的岗位人才评测模型，并对各要素数据量化、权重分配进行深入研究。。

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简介：摘要：本文首先分析了汽车4S店可持续性发展的必要条件，并研究了汽车4S店可持续性发展的有效途径，首先是要打造业务精进的销售团队，其次要通过建立服务标准、加强配件质量管理、注重服务设施等方式提高售后服务质量，另外要选对赛道，关注企业文化、品牌效应以及汽车4S店的建筑设计。

简介：摘要：在我国可持续发展理念深入人心的大背景下，植物研究行业的专家加大了研究力度，为促进我国生物多样化发展做出了卓越贡献。本文以河北衡水湖国家级自然保护区的李、秋子梨、稠李、榆叶梅四种常见蔷薇科植物为例，对植物萌芽时期、展叶时期、开花时期、结果时期的物候变化情况进行观测，探究分析了不同时期植物生长的影响因素，致力于为我国植物培育工作提供参考意见。

简介：摘要：无线网络参数配置直接影响终端用户使用感知，因此，对网络参数的有效管理至关重要。为了确保良好的4G/5G服务质量，无线工程师必须熟悉参数原理以网管参数配置。由于4G/5G服务提供的网络连接数量多，因此手动执行大量参数管理会带来巨大工作量。随着网络流量与用户需求的不断变化，运营商/设备商对用户体验的需求也在提高，需要一种智能高效的参数管理方法确保无线网络服务质量。

简介：摘要：本文首先分析了汽车4S店可持续性发展的必要条件，并研究了汽车4S店可持续性发展的有效途径，首先是要打造业务精进的销售团队，其次要通过建立服务标准、加强配件质量管理、注重服务设施等方式提高售后服务质量，另外要选对赛道，关注企业文化、品牌效应以及汽车4S店的建筑设计。

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简介：摘要：当代科学技术的发展和进步在给人们带来了诸多便利的同时也为人们提供了了解这个世界的渠道，在不久的将来4G通信工程技术将会对人们的生活产生更加深远的影响。

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简介：摘要：随着移动通信技术和移动智能终端的快速发展，使人们对移动网络的需求猛增，4G无线网络的出现可以充分满足人们这方面的需求。近年来，运营商在4G无线网络建设方面取得了长足的进步，但仍有很多地方没有深度覆盖。下面就如何推广和优化4G无线网络进行分析和说明。

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简介：AbstractAfrican swine fever (ASF) is a highly infectious, transboundary viral disease of domestic and wild pigs, and is currently the most serious threat to world swine production, resulting in significant economic loss. In the absence of vaccines and treatments, the control of the disease entirely depends on accurate and early diagnosis accompanied by the culling of infected pigs. Thus, a highly specific and sensitive diagnostic assay is required during an outbreak and surveillance of the disease. In this study, a highly sensitive, specific, rapid and repeatable P22-monoclonal antibody-based blocking enzyme-linked immunosorbent assay (bELISA) assay was developed for the detection of antibodies against genotype I and II African swine fever viruses(ASFVs). A total of 806 pig serum samples were tested to evaluate the performance of the diagnostic assay. To determine the PI (percent Inhibition) cut-off value, receiver-operating characteristic (ROC) analysis was applied. According to the ROC analysis of the data, 98.10% specificity and 100% sensitivity were recorded when the threshold cut-off value of PI was established at 47%. In addition, the assay was able to detect ASFV antibodies as early as 9 days post-infection when serum samples from experimentally infected pigs were used. Taking all together, the results of the present study indicated that the P22-mAb based bELISA assay can be used for rapid and accurate detection of antibodies against ASFV, which could play a valuable role in the containment and prevention of ASFV as an alternative to other serological diagnostic methods. Also, this study will assist researchers to further investigate the immunogenic importance of P22 protein in ASFV infection.

简介：摘要目的探讨微小RNA（miRNA）-4795-3p通过靶基因表皮生长因子受体（epidermal growth factor receptor，EGFR）抑制胃癌细胞增殖和侵袭。方法本研究于2020年6月至12月分别转染阴性对照模拟物（阴性对照组）和miR-4795-3p模拟物（miR-4795-3p组）至胃癌BGC823细胞。实时定量聚合酶链反应（qRT-PCR）检验转染效率。淋巴细胞增殖检测（MTS）法和Transwell实验检测BGC823细胞的增殖情况及侵袭能力。生物信息学软件miRcode预测miR-4795-3p的靶基因，采用双荧光素酶报告基因实验验证miR-4795-3p与靶基因的结合。qRT-PCR和蛋白质免疫印迹试验（Western blot）检测靶基因的表达。采用SPSS 21.0软件分析数据，组间比较应用独立样本t检验，P<0.05为差异有统计学意义。结果miR-4795-3p组和阴性对照组BGC823细胞中miR-4795-3p表达分别为（1.02±0.11）和（11.04±1.23），阴性对照组miR-4795-3p表达明显低于miR-4795-3p组（t=8.14，P<0.01）。与阴性对照组比较，miR-4795-3p组BGC823细胞吸光度值明显下降（P<0.05），细胞侵袭数明显减少（P<0.01）。miR-4795-3p与EGFR存在互补结合位点，miR-4795-3p可互补结合EGFR（P<0.01）。与阴性对照组比较，miR-4795-3p组BGC823细胞中EGFR表达明显降低（P<0.01）。结论miR-4795-3p通过靶向负调控EGFR抑制胃癌BGC823细胞的增殖和侵袭能力。

简介：AbstractBackground:Gene promoter methylation is a major epigenetic change in cancers, which plays critical roles in carcinogenesis. As a crucial regulator in the early stages of B-cell differentiation and embryonic neurodevelopment, the paired box 5 (PAX5) gene is downregulated by methylation in several kinds of tumors and the role of this downregulation in esophageal squamous cell carcinoma (ESCC) pathogenesis remains unclear.Methods:To elucidate the role of PAX5 in ESCC, eight ESCC cell lines, 51 primary ESCC tissue samples, and eight normal esophageal mucosa samples were studied and The Cancer Genome Atlas (TCGA) was queried. PAX5 expression was examined by reverse transcription-polymerase chain reaction and western blotting. Cell apoptosis, proliferation, and chemosensitivity were detected by flow cytometry, colony formation assays, and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assays in ESCC cell lines with PAX5 overexpression or silencing. Tumor xenograft models were established for in vivo verification.Results:PAX5 methylation was found in 37.3% (19/51) of primary ESCC samples, which was significantly associated with age (P = 0.007) and tumor-node-metastasis stage (P = 0.014). TCGA data analysis indicated that PAX5 expression was inversely correlated with promoter region methylation (r = -0.189, P = 0.011 for cg00464519 and r = -0.228, P = 0.002 for cg02538199). Restoration of PAX5 expression suppressed cell proliferation, promoted apoptosis, and inhibited tumor growth of ESCC cell lines, which was verified in xenografted mice. Ectopic PAX5 expression significantly increased p53 reporter luciferase activity and increased p53 messenger RNA and protein levels. A direct interaction of PAX5 with the p53 promoter region was confirmed by chromatin immunoprecipitation assays. Re-expression of PAX5 sensitized ESCC cell lines KYSE150 and KYSE30 to fluorouracil and docetaxel. Silencing of PAX5 induced resistance of KYSE450 cells to these drugs.Conclusions:As a tumor suppressor gene regulated by promoter region methylation in human ESCC, PAX5 inhibits proliferation, promotes apoptosis, and induces activation of p53 signaling. PAX5 may serve as a chemosensitive marker of ESCC.