简介:Heatshockproteins(HSPs),suchasHSP70andHSP90,areasuiteofhighlyconservedproteinsproducedinallcellularorganismswhentheyareexposedtostresses.Inaquaticanimals,theyhavebeenprovedtoplayimportantrolesinresponsetoenvironmentalpollutantsandparticularlyinthenon-specificimmuneresponsestopathogeninfections.Inthepresentstudy,theexpressionprofilesofHSP70andHSP90genesinhemocytesandhepatopancreasfromtheridgetailwhiteprawnExopalaemoncarinicaudainfectedwithWSSVandVibrioanguillarumweredetectedusingreversetranscriptionpolymerasechainreaction(RT-PCR).AfterWSSVchallenge,theexpressionlevelofHSP70genetranscriptsinthehemocytesandhepatopancreasincreasedtopeaklevelat6hand48h,respectively.HSP90genetranscriptsinhemocytesandhepatopancreaswereup-regulatedsignificantlyat12hand6h,respectively.DuringV.anguillarumchallenge,themRNAcontentofHSP70geneinhemocytesandhepatopancreasincreasedsignificantlyat12hand6hpost-infection,respectively.TheexpressionlevelofHSP90genebothinhemocytesandhepatopancreaswereup-regulatedinthefirst3h.TheexpressionpatternsofHSP70andHSP90genesinhemocytesandhepatopancreasshowedtemporalandspatialdifferencesafterchallengedwithWSSVandV.anguillarum.TheresultssuggestedthatHSPsmightbeinvolvedinimmuneresponsestopathogenschallengeinE.carinicauda.
简介:以卤虫无节幼体(36h)和成虫(16d)为例,在温度为15±3℃、盐度为30的条件下,通过单因子静态急性毒性试验方法,分别研究了Cu2+、Mn2+和Zn2+对卤虫的无节幼体和成虫的单一毒性效应;在此基础上,以卤虫卤虫无节幼体(36h)为例,在相同试验条件下,通过等毒性溶液法,研究了Cu2+、Mn2+和Zn2+对卤虫的联合毒性效应。试验结果表明:卤虫无节幼体对三种金属离子的耐受能力均高于成虫,Cu2+﹑Mn2+和Zn2+的48hLC50分别是4.0、27.19和115.95mg/L,而成虫Cu2+﹑Mn2+和Zn2+的48hLC50仅为1.31、6.86和34.23mg/L;且对两个时期的卤虫,三种金属离子毒性由强至弱均为Cu2+>Mn2+>Zn2+;三种重金属对卤虫无节幼体和成虫的安全浓度(SC)分别是0.4、2.72、11.6、0.13、0.69和3.42mg/L。联合毒性试验结果表明Cu-Mn、Cu-Zn和Mn-Zn均对卤虫无节幼体表现为拮抗作用。
简介:对1998年6月南海北部20天的海流和温度定点连续观测资料进行分析,得到该海域内潮的特征及其能量分布。分析结果显示内潮的主要成分为O1,K1,M2与S2分量,其中全日内潮(O1与K1)的能量占主要部分。在观测期间,此四个分量的海流失量均为顺时针旋转,其潮流椭圆半长轴的最大值超过14cm/s。海水温度的变化显示出内潮存在准日周期振动,平均垂向振幅达到50m。观测到的内潮携带高能量且其活动存在不连续性,在观测范围内,全日内潮的动能及势能密度的最大值分别达到2kJ/m^2及3.5kJ/m^2,半日内潮的动能及势能密度的最大值分别达到1kJ/m^2及1.5kJ/m^2。