简介：目的methotrexate(MTX)的最重要的副作用是mucositis。这研究的目的是在收到methotrexate的老鼠在肠的损坏和氧化应力上评估沉金摘录的效果。方法实验在划分成六个组的男Wistar白化体老鼠上被执行。首先组织收到的正常盐口头上地，第二个组收到了沉金摘录(100mg瑡潩?????????????????假吗？？

简介：ThepresentstudywasdesignedtodeterminetheeffectsofatraditionalChinesemedicine,calledQishenYiqiDroppingPillonchronichypoxia-inducedmyocardialinjury.ToestablisharatchronichypoxiamodeltobeusedintheevaluationofthetherapeuticeffectsoftheQishenYiqiDroppingPill,Sprague-Dawley(SD)ratswererandomlydividedintothreegroups:thecontrol,model,andtreatmentgroups(n=10pergroup).Theanimalswerehousedinaplexiglasscontainer.Thecontrolanimalswereundernormaloxygenconcentrationandthemodelandtreatmentgroupswereexposedtoairandnitrogenfor5weeks.TheratsinthetreatmentgroupwereorallyadministeredtheQishenYiqiDroppingpill(35mg·kg-1·d-1)for5weeks.Afterthetreatment,thecardiacfunctionandmorphologywereanalyzed,andtheexpressionlevelsofhypoxia-induciblefactor1α(HIF-1α)weredeterminedusingWesternblotting.Ourresultsindicatedthatthecardiacfunctionwasimpaired,cellapoptosiswasenhanced,andHIF-1αexpressionwasup-regulatedinthemodelgroup,comparedtothecontrolgroup.ThesechangeswereamelioratedbythetreatmentwiththeQishenYiqiDroppingPill.Inconclusion,QishenYiqiDroppingpillcanamelioratemyocardialinjuryinducedbychronichypoxia,improvecardiacfunction,anddecreasemyocardialcellapoptosis,whichmayprovideabasisforitsclinicaluseforthetreatmentofchroniccardiovasculardiseases

简介：Pleurotussajorcaju(P。sajorcaju)，一朵可食、无毒的蘑菇，作为抗氧化剂被评估，antitumor，反煽动性并且antihypertensive活动。P。sajorcaju是糖类，饮食的纤维，必要氨基酸，矿物质，维生素B，folic酸和类固醇的好来源。反煽动性，immunomodulatory和止痛活动水并且P的菌丝体的methanolic摘录。sajorcaju被调查(数据没被显示出)。这发现建议那P提取。sajorcaju能对煽动性、自体免疫的疾病被使用。那么，P。为它的antiarthritic活动检验的sajorcaju。植物与水和甲醇镇定、独立提取。为antiarthritic活动500和1000mg

简介：

简介：Cyclophosphamide(CP)广泛地为各种各样的癌症，以及一个抑制免疫力的代理人的治疗被使用。然而，CP被知道包括繁殖毒性引起几不利效果。Achilleamillefolium，广泛地分布式的药用的植物，高度为它的药用的活动，包括的抗氧化剂和反煽动性的性质被考虑。现在的学习被进行估计是否有抗氧化剂和反煽动性的活动的水的摘录能在CP治疗期间对繁殖毒性作为一个保护的代理人服务的Achilleamillefolium开花期。男Wistar老鼠被分成四个组。二组老鼠是在5mg的剂量的管理CP?????????????????吗？？

简介：Sheng-Mai-San(SMS),awell-knownChinesemedicinalplantformula,iswidelyusedforthetreatmentofcardiacdiseasescharacterizedbydeficiencyofQiandYinsyndrome.Amousechronicintermittenthypoxia(CIH)modelwasestablishedtomimictheprimaryclinicalfeaturesofdeficiencyofQiandYinsyndrome.MiceexperiencedCIHfor28days(nadir7%topeak8%oxygen,20minperday),resultinginleftventricle(LV)dysfunctionandstructureabnormalities.AfteradministrationofSMS(0.55,1.1,and5.5g·kg-1·d-1)forfourweeks,improvedcardiacfunctionwasobserved,asindicatedbytheincreaseintheejectionfractionfromtheLVonechocardiography.SMSalsopreservedthestructuralintegrityoftheLVagainsteccentrichypotrophy,tissuevacuolization,andmitochondrialinjuryasmeasuredbyhistology,electronmicroscopy,andultrasoundassessments.Mechanistically,theantioxidanteffectsofSMSweredemonstrated;SMSwasabletosuppressmitochondrialapoptosisasindicatedbythereductionofseveralpro-apoptoticfactors(Bax,cytochromec,andcleavedcaspase-3)andup-regulationoftheanti-apoptosisfactorBcl-2.Inconclusion,theseresultsdemonstratethatSMStreatmentcanprotectthestructureandfunctionoftheLVandthattheprotectiveeffectsofthisformulaareassociatedwiththeregulationofthemitochondrialapoptosispathway.

简介：

简介：ThepresentstudywasdesignedtoevaluatetheprotectiveeffectsofethanolextractsofRabdosiajaponicavar.glaucocalyx(Maxim.)Hara(RJ)onlipopolysaccharide(LPS)-inducedacutelunginjury(ALI)inmiceandthepossibleunderlyingmechanismsofaction.ThemicewereorallyadministratedwithRJextract(16,32or64mg?kg–1)dailyforconsecutive7daysbeforeLPSchallenge.Theungspecimensandthebronchoalveolarlavagefluid(BALF)werecollectedforhistopathologicalexaminationsandbiochemicalanalyses.PretreatmentwithRJsignificantlyenhancedsuperoxidedismutase(SOD)activityandreducedthewet-to-dryweight(W/D)ratio,thelevelsofnitricoxide(NO)andproteinleakage,andmyeloperoxidase(MPO)activityinmicewithALI,inadose-dependentmanner.RJreducedcomplementdepositionandsignificantlyattenuatedLPS-inducedALIbyreducingproductionsofinflammatorymediators,suchastumornecrosisfactor-α(TNF-α),interleukin-6(IL-6),andinterleukin-1β(IL-1β).TheresultsdemonstratedthatRJmayattenuateLPS-inducedALIviareducingtheproductionofpro-inflammatorymediators,andreducingcomplementdepositionandradicals.

简介：PhyllanthusUrinariaL.(PUL)isatraditionalChinesemedicineusedtotreathepaticandrenaldisorders.However,themechanismofitshepatoprotectiveactionisnotfullyunderstood.Inthepresentstudy,bloodbiochemicalindexesandliverhistopathologicalchangeswereusedtoestimatetheextentofhepaticinjury.GC/MSandLC/MS-baseduntargetedmetabolomicswereusedincombinationtocharacterizethepotentialbiomarkersassociatedwiththeprotectiveactivityofPULagainstCCl_4-inducedliverinjuryinrats.PULtreatmentcouldreversetheincreaseinALT,ASTandALPinducedbyCCl_4andattenuatethepathologicalchangesinratliver.SignificantchangesinlivermetabolicprofilingwereobservedinPUL-treatedgroupcomparedwithliverinjurymodelgroup.SeventeenbiomarkersrelatedtothehepatoprotectiveeffectsofPULagainstCCl_4-inducedliverinjurywerescreenedoutusingnonparametrictestandPearson'scorrelationanalysis(OPLS-DA).TheresultssuggestedthatthepotentialhepatoprotectiveeffectsofPULinattenuatingCCl_4-inducedhepatotoxicitycouldbepartiallyattributedtoregulatingL-carnitine,taurocholicacid,andaminoacidsmetabolism,whichmaybecomepromisingtargetsfortreatmentoflivertoxicity.Inconclusion,thisstudyprovidesnewinsightsintothemechanismofthehepatoprotectionofPhyllanthusUrinaria.

简介：Benignprostatichyperplasia(BPH)isanage-relateddiseaseofunknownetiology,characterizedbyprostaticenlargementcoincidentwithdistinctalterationsintissuehistology.Inthepresentstudy,weinvestigatedwhethertriptolidecanpreventtestosterone-inducedprostatichyperplasiainrats.Castrationwasperformedviathescrotalrouteafterurethaneaesthesia.BPHwasinducedinexperimentalgroupsbydailysubcutaneousinjectionsoftestosteronepropionate(TP)fortwoweeks.Triptolidewasadministereddailybyoralgavageatadoseof100and50μg×kg~(-1)for2weeks,alongwiththeTPinjections.Onday14,theanimalswerehumanelykilledbycervicaldislocationafteraesthesia.Prostateswereexcised,weighed,andusedforhistologicalstudies.Testosteroneanddihydrotestosterone(DHT)levelsinserumandprostateweremeasured.Theresultsshowedthattriptolidesignificantlyreducedtheprostateweight,andthetestosteroneandDHTlevelsinboththeserumandprostate.HistopathologicalexaminationalsoshowedthattriptolidetreatmentsuppressedTP-inducedprostatichyperplasia.Inconclusion,triptolideeffectivelyinhibitsthedevelopmentofBPHinducedbytestosteroneinaratmodel.

简介：在hematological上调查Ipomoeacarnea花的乙醇摘录的活跃氯仿部分的目的在Wistar老鼠在甲苯改变导致diisocyanate的发炎。方法除了控制组，，所有用10%甲苯diisocyanate(TDI)的5L的intranasal申请老鼠被敏化7天。在第二促进感受性以后的一个星期，所有与5%TDI的5L老鼠被挑起导致航线超敏性。在最后挑战以后，血和bronchoalvelorlavage(BAL)液体镇定、使遭到总计，微分白细胞数。闪光层析在最活跃的氯仿部分上被执行孤立一个单个部件。有在200mg摥琠敨洠獯?汰畡楳汢?潳牵散漠?畨慭?湩敦瑣潩?的口头的剂量的ethanolic摘录和它的氯仿部分的结果处理??桴?慳敭瀠牥潩?挠獡獥漠?L牯楴湯挠畡敳?祢??畢湲瑥楩眠牥?潣普物敭?瑡琠潷搠楡祲猠敨灥映牡獭?匊湩散㈠??愠氠牡敧洠汵楴楤'諡箻H慮祲爠獥慥捲?潰瑲潦楬?慨?瑳牡整?愠浩摥愠?敧敮慲楴杮戠瑥整?湫睯敬杤?L畯?桴獩搠獩慥敳?湉?湵?????敦敶?湩猠慭汬爠浵湩湡獴欠灥?潦?業歬瀠潲畤瑣潩?敢慣敭渠瑯晩慩汢?湩琠敨丠瑥敨汲湡獤映牯映牡獭眠瑩?湡愠潢瑲潩?慲整漠?潭敲琠慨?楦敶瀠牥挠湥???桴?畡畴湭漠

简介：Ginkgolicacids(GAs),primarilyfoundintheleaves,nuts,andtestaofginkgobiloba,havebeenidentifiedwithsuspectedallergenic,genotoxicandcytotoxicproperties.However,littleinformationisavailableaboutGAstoxicityinkidneysandtheunderlyingmechanismhasnotbeenthoroughlyelucidatedsofar.InsteadofGAsextract,therenalcytotoxicityofGA(15:1),whichwasisolatedfromthetestaofGinkgobiloba,wasassessedinvitrobyusingMDCKcells.TheactionofGA(15:1)oncellviabilitywasevaluatedbytheMTTandneutralreduptakeassays.Comparedwiththecontrol,thecytotoxicityofGA(15:1)onMDCKcellsdisplayedatime-anddose-dependentmanner,suggestingthecellsmitochondriaandlysosomesweredamaged.ItwasconfirmedthatGA(15:1)resultedinthelossofcellsmitochondrialtrans-membranepotential(ΔΨm).Inpropidiumiodide(PI)staininganalysis,GA(15:1)inducedcellcyclearrestattheG0/G1andG2/Mphases,influencingontheDNAsynthesisandcellmitosis.CharacteristicsofnecroticcelldeathwereobservedinMDCKcellsattheexperimentalconditions,asaresultofDNAagarosegelelectrophoresisandmorphologicalobservationofMDCKcells.Inconclusion,thesefindingsmightprovideusefulinformationforabetterunderstandingoftheGA(15:1)inducedrenaltoxicity.

简介：ScutellariaeRadix(SR),therootofScutellariabaicalensisGeorgi,isusedasanantipyreticdrugandhasbeendemonstratedtohaveanti-inflammatoryactivity.SRisdividedintotwospecifications,'KuQin'(KQ)and'ZiQin'(ZQ),foruseagainstdifferentsymptoms(upperenergizerheatorlowerportionofthetripleenergizer),accordingtothetheoryoftraditionalChinesemedicine(TCM).However,differencesintheefficaciesofthesetwospecificationshavenotbeendetermined.Inthepresentstudy,weaimedtocharacterizethedifferencesintheanti-inflammatoryactivitiesbetweenKQandZQandtoexplorehowtheirdifferencesaremanifestedinlipopolysaccharide(LPS)-inducedmacrophages.Ourresultsshowedthat,inRAW264.7cells(amousemacrophagecelllinederivedfromascites),KQandZQdisplayedanti-inflammatoryeffectsbyinhibitingthereleaseofnitricoxide(NO),inducibleNOS(iNOS),andnuclearfactor-κB(NF-κB)inadose-dependentmannerwithoutdistinction.InNR8383cells(aratalveolarmacrophagecellline),KQandZQdisplayedsimilareffectsonNO,iNOS,andNF-κBasseeninRAW264.7cells,butKQshowedahigherinhibitionrateforNOandiNOSthanthatshownbyZQatthesameconcentration.TheseresultsindicatedthatthereweredifferencesinefficacybetweenKQandZQintreatinglunginflammation.OurfindingsprovidedanexperimentalevidencesupportingthedifferentusesofKQandZQinclinic,asnotedinancientherbalrecords.

简介：Theuterinetetaniccontractionanduterinearterybloodflowreductionarepossiblereasonsforprimarydysmenorrhea（PD）.Inthepresentstudy,weaimedtoevaluatetheuterinerelaxanteffectandtheinfluenceonuterinearterybloodvelocityofGe-GenDecoction（GGD）,awell-knownChineseherbalformula.InfemaleICRmice,uterinecontractionwasinducedbyoxytocinexposurefollowingestradiolbenzoatepretreatment,andtheuterinearterybloodvelocitywasdetectedbyDopplerultrasound.HistopathologicalexaminationoftheuterinetissuesampleswereperformedbyH&Estaining.Exvivostudiesdemonstratedthatoxytocin,posteriorpituitary,oracetylcholineinducedcontractionsinisolatedmouseuterus.GGDinhibitedbothspontaneousandstimulatedcontractions.InvivostudydemonstratedthatGGDsignificantlyreducedoxytocin-inducedwrithingresponseswithamaximalinhibitionof87%.FurtherstudydemonstratedthatGGDnormalizedoxytocin-inducedabnormalitiesofprostaglandinsF2alpha(PGF2α)andCa2+inmice.Inaddition,injectionofoxytocininducedadecreaseinuterinearterybloodflowvelocity.PretreatmentwithGGDreversedtheoxytocinresponseonbloodflowvelocity.HistopathologicalexaminationshowedpretreatmentwithGGDalleviatedinflammationandedemaintheuteruswhencomparedwiththemodelgroup.BothexvivoandinvivoresultsindicatedthatGGDpossessedasignificantspasmolyticeffectonuterinetetaniccontractionaswellasimprovementonuterinearterybloodvelocitywhichmayinvolvePGF2αandCa2+signaling,suggestingthatGGDmayhaveaclinicpotentialinPDtherapy.

简介：介绍Cystone是为各种各样的尿混乱在印度使用的同意的Ayurvedicpolyherbal专卖药品，包括urolithiasis。试图在urolithiasis把Cystone的保护的效果与导致hyperoxaluria的氧化应力和钙盐水晶免职作比较。方法乙烯乙二醇(例如)(0.75%，V/V)在喝，水被给老鼠28天与Cystone(500和750mg/kg身体重量)的同时的处理导致urolithiasis，并且urolithiasis的各种各样的尿风险因素和抗氧化剂标记被估计。例如结果处理导致增加的尿体积和降低的尿pH与盐的增加的尿排泄一起，在未经治疗的动物的钙和磷酸盐。这些变化在未经治疗的老鼠的肾引起了广泛的钙盐水晶免职，增加的类脂化合物peroxidation和抗氧化剂酶(草皮，过氧化氢酶和GPx)的减少的活动。Cystone阻止了这些hyperoxaluric表明并且在两剂量在对待的老鼠禁止了钙盐水晶免职。结论Cystone治疗由改进肾的织物抗氧化剂地位和多尿对导致hyperoxaluria的氧化应力和钙盐水晶免职提供保护。

简介：Capsaicin(trans-8-methyl-N-vanillyl-6-nonenamide)isthemaincomponentinhotpeppers,includingredchilipeppers,alapenos,andhabanero,belongingtothegenusCapsicum.Capsaicinisapotentantioxidantthatinterfereswithfreeradicalactivities.Inthepresentstudy,thepossibleprotectiveeffectofcapsaicinwasstudiedagainstmethylmethanesulphonate(MMS)inducedtoxicityinthirdinstarlarvaeoftransgenicDrosophilamelanogaster(hsp70-lacZ)Bg~9.ThethirdinstarwasallowedtofeedonthediethavingdifferentdosesofcapsaicinandMMSseparatelyandincombination.TheresultssuggestedthattheexposureofthirdinstarlarvaetothediethavingMMSaloneshowedsignificanthsp70expressionaswellastissueDNAandoxidativedamage,whereasthelarvaefeedonthediethavingMMSandcapsaicinshowedadecreaseinthetoxiceffectsfor48-hofexposure.Inconclusion,capsaicinshowedadose-dependentdecreaseinthetoxiceffectsinducedbyMMSinthethirdinstarlarvaeoftransgenicDrosophilamelanogaster.

简介：ThefruitsofPaulowniacatalpifoliaGongTongareusedasaChinesefolkherbalmedicineforthetreatmentofenteritis,tonsillitis,bronchitis,anddysentery,etc.OurpreviousstudyhasidentifiednewC-geranylatedflavanoneswithobviousanti-proliferativeeffectsinlungcancerA549cells.Inthepresentstudy,anewC-geranylatedflavone,paucatalinoneC(1)andfiveknownC-geranylatedflavanones(2-6)wereisolated.Inaddition,atotalof34C-geranylatedflavonoidsweredetectedbyHPLC-DAD-ESI-MS/MScouplingtechniquesfromtheCH_2Cl_2extractofP.catalpifolia.Futhermore,anti-agingeffectsofisolatedcompoundswereevaluatedinvitrowithprematuresenescent2BScellsinducedbyH_2O_2.PhytochemicalresultsindicatedthatP.catalpifoliawasanaturalresourceofabundantC-geranylatedflavonoids.Diplacone(3)andpaucatalinoneA(5)werethepotentanti-agingagentsintheprematuresenescent2BScellsinducedbyH_2O_2andtheC-geranylsubstituentmaybeanimportantfactorbecauseofitslipophiliccharacter.

简介：Thepresentstudywasdesignedtosynthesize2-Cyano-3,12-dioxooleana-1,9(11)-en-28-oate-13β,28-olide(1),alactonederivativeofoleanolicacid(OA)andevaluateitsanti-inflammatoryactivity.Compound1significantlydiminishednitricoxide(NO)productionanddown-regulatedthemRNAexpressionofiNOS,COX-2,IL-6,IL-1β,andTNF-αinlipopolysaccharide(LPS)-stimulatedRAW264.7cells.FurtherinvivostudiesinmurinemodelofLPS-inducedacutelunginjury(ALI)showedthat1possessedmorepotentprotectiveeffectsthanthewell-knownanti-inflammatorydrugdexamethasonebyinhibitingmyeloperoxidase(MPO)activity,reducingtotalcellsandneutrophils,andsuppressinginflammatorycytokinesexpression,andthusamelioratingthehistopathologicalconditionsoftheinjuredlungtissue.Inconclusion,compound1couldbedevelopedasapromisinganti-inflammatoryagentforinterventionofLPS-inducedALI.