简介：ObjectToinvestigatetheclinicalsignificanceofallogeneichematopoieticstemcelltransplantation(allo-HSCT)followingfludarabine(Flu)-basednomnyeloablativeconditioningregimen.Methods7patientswitholderageororgandysfunctionreceivedeitheroftwoFlu-basednonmyeloablativeconditioningprotocolsfollowedbyinfusionofgranulocytecolony-stimulatingfactor(G-CSF)mobilizedallogeneicperipheralblooclstemcells(PBSC).Cy-elosporincombiningmethotrexatewasusedasgraftvshostdisease(GVHD)prophylaxis.ResultsMinimalalloHSCTassociatedtoxicitywasfoundapartfrommucositis.Theallogeneicdonorengraftmentswereverifiedinallthepatients.Sixofseveneasessurvivedmorethan5months.AcuteGVHDoccurredinthreeofsevenpatientsincludingacaseofgrade1IGVHD.ConclusionTherapidengrafunentofallo-PBSCandgraftvsleukemia(GVL)effectscanbeobtainedbyFlu-basednomnyeloablativeconditioningregimen.Thismanagementissuitableforthepatientswhoaretoooldorhaveorgandysfunction.

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简介：Objective:Toobservetheeffectsofcryopreservedolfactoryensheathingcells(OECs)transplantationonaxonalregenerationandfunctionalrecoveryfollowingspinalcordinjuryinadultrats.Methods:Twenty-fourratsweredividedintoexperimentalandcontrolgroups,eachgrouphaving12rats.ThespinalcordinjurywasestablishedbytransectingthespinalcordatT10levelwithmicrosurgeryscissors.OECswerepurifiedfromSDratolfactorybulbandculturedinDMEM(Dulbecco'sminimumessentialmedium)andcryopreserved(-120℃)fortwoweeks.OECssuspension[(1-1.4)×105/ul]wastransplantedintotransectedspinalcord,whiletheDMEMsolutionwasinjectedinsteadinthecontrolgroup.At6and12weeksaftertransplantation,theratswereevaluatedwithclimbingtestandMEP(moterevokedpotentials)monitoring.Thesamplesofspinalcordwereprocuredandstudiedwithhistologicalandimmunohistochemicalstainings.Results:At6weeksaftertransplantation,alloftheratsinbothtransplantedandcontrolgroupswereparaplegic,andMEPscouldnotberecorded.MorphologyoftransplantedOECswasnormal,andOECswereinterfusedwithhostwell.Axonscouldregrowintogaptissuebetweenthespinalcords.BothOECsandregrownaxonswereimmunoreactiveforMBP.Noregrownaxonswerefoundinthecontrolgroup.At12weeksaftertransplantation,2rats(2/7)hadlowerextremitiesmusclecontraction,2rats(2/7)hadhipand/orkneeactivemovement,andMEPof5rats(5/7)couldberecordedinthecalfinthetransplantationgroup.Noneoftherats(7/7)inthecontrolgrouphadfunctionalimprovement,andnonehadMEPsrecorded.Inthetransplantedgroup,histologicalandimmunohistochemicalmethodsshowedthenumberoftransplantedOECsreducedandsomeregrownaxonshadreachedtheendoftransectedspinalcord.However,noregrownaxonscouldbeseenexceptscarformationinthecontrolgroup.Conclusions:CryopreservedOECscouldintegratedwiththehostandpromoteregrowingaxonsacrossthetransectedsp

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简介：许多癌症都依赖于EGFR（表皮生长因子受体）来维持旺盛生长。一些被期望通过关闭这一细胞信号接收位点来起作用的药物,却在大多数患者中没有能够阻止住癌症。在正常细胞中,EGFR发挥功能可使细胞受益。然而在大量的癌症中--从较常见的卵巢癌和皮肤癌到较少见的侵袭性癌症如大脑胶质母细胞瘤--控制EGFR的基因往往被重编程,并生成了太多的EGFR,由此导致了失控性的生长以及癌细胞扩散。

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简介：Objective:Toevaluatetheefficacyoffreetransplan-tationofdenervatedmusclesandvesselsinthetreatmentoflong-standingfacialparalysis.Methods:Atotalof26patientswithfacialparalysis(10malesand16females,aged16-65years,mean:47years)wereenrolledinthisstudytoreceivetransplantationofdenervatedextensordigitorumbrevis(EDB)andextensorhallusisbrevis(EHB).Themuscletendonwasslungtothealanasi,themiddlepointofthenasolabialsulcus,theangu-lusotisandthechintocorrectthenasalandoraldeformity.Themusclebellywasburiedaroundthenervesthatinner-vatedthemassetermuscle.Microsurgerywasappliedtoanastomosingthetarsuslateralvesselstothesuperficialtemporalisvessels.Results:Afteroperation,allthepatientsimmediatelyobtainedsatisfiedstaticappearance.Themovementoftheparalyzedcomerofthemouthcouldbeobtainedonemonthlaterandthesmileoftheparalyzedsidecouldberestoredafter3monthsoftraining.And88%patientsachievedper-fectresults,8%obtainedsatisfactoryresults,and4%gotimprovement6monthsafteroperationaccordingtoStennert'sparesisscoringsystem.Conclusions:Freetransplantationofdenervatedmusclesandvesselsforthetreatmentoflong-standingfa-cialparalysis,whichseldomcausesatrophyorliquefactionofthetransferredmuscles,canmaintainmuscleviabilityandinducereliablenerveregeneration.Therefore,itisasafeandefficienttreatmentmethodforthepatientssuffer-ingfromfacialparalysis.

简介：Objective:Toprovideasoundcellsourceforfurtherex-vivogenetherapyforchronicpain,weattempttodevelopanimmortalizedratastrocytecelllinethatexpressesenkephalinregulatedbydoxycycline.Methods:Retrovirusinfectionmethodwasemployedtodevelopanimmortalizedratastrocytecelllinethatcouldexpressenkephalinregulatedbydoxycycline.ThehPPEgeneexpressionlevelofimmoralizedastroytecells(IAC)/hPPEwasdetectedbyRT-PCR,indirectimmunofluorescencestainingandradioimmunoassay.Results:IACcarryingTet-onsystemtransfectedwithpreproenkephalingenecouldsecreteenkephalinthatwasregulatedbydoxycyclineinadose-dependentmannerandhPPEgeneactivationcouldberepeatedinon-off-oncyclesthroughadministrationorremovalofdoxycycline.Conclusion:Animmortalizedratastrocytecelllinethatsecreteenkephalinunderthecontrolofdoxycyclineisestablishedsuccessfully,whichprovidesaresearchbasisfortransgeniccelltransplantationforanalgesia.

简介：Aim:Toinvestigateglycoconjugatechangesonthecellsurfaceofproliferativelesionsandneoplasmsofmicelungsatvariousstagesoftumorigenesis,therelationbetweenprogressivedevelopmentofmousepulmonarytumorsandexpressionofcellsurfacesaccharide.Materialsandmethods:Thirty-onemaleA/Jstrainmiceat5weeksofageweretreatedintraperitoneallywithasingleinjectionof20-methylcholanthrene(20-MC),292pulmonarylesionsincluding31hyperplasias,145alveolaradenomas,61papillaryadenomas,55papillaryadenocarcinomasandtheircombinedtypewereobtained.Thebindingaffinitiesofcellsinnormalrespiratoryepitheliaandinproliferativelesionstofourperoxidases-conjugatedlectins,Maclurapomiferaagglutinin(MPA),Arachishypogeaagglutinin(PNA),Ricinuscommunisagglutinin(RCA),andwheatgermagglutinin(WGA)wereexamined.Results:Cellsofhyperplasiaandalveolaradenomashowedfairlystrongaffinitytoallthefourlectins.However,partofpapillaryadenomacellsandgreaterpartofpapillaryadenocarcinomacellslosttheirbindingaffinitytoMPA,PNA,andRCA,butnottoWGA.ThebindingsofMPA,PNAandRNAweredetectedpredominentlyontheluminalsurfacesofbenigntumorsbutnotontheluminalsurfacesofmalignanttumors.WGAmightbindtovariedtypesofbenignandmalignanttumors.Pretreatedwithneuraminidase,thelesionsenhancedthestainingintensityforthefourlectins,thebindingsitesofWGAtomalignanttumorcellswerenumerous.Adistinctdifferenceinlectinbindingaffinitybetweenhyperplasia/alveolaradenoma/papillaryadenomaandpapillaryadenocarcinomawasclearlyshown(x2=46.89,P＜0.01,x2=36.77,P＜0.01andx2=52.87,P＜0.01)inthisexperiment.Thecomplexglycoconjugatesonthecellsurfaceofmalignantandbenignlesionsduringthedevelopmentofpulmonarytumorwerechanged,malignanttumorcellsdifferedfromthesurfaceofbenigntumorcells,thelevelsoftotalsialicacidwerehigherinmalignanttumorce

简介：Objective:Toexplorethecultureconditionsofhumanneuralstemcellsandtoinvestigatetheultrastructureofneurospheres.Methods:Thecellsfromtheembryonichumancorticesweremechanicallydissociated.N2mediumwasadaptedtocultureandexpandthecells.ThecellswereidentifiedbyimmunocytochemistryandEMwasappliedtoexaminetheultrastructureofneurospheres.Results:Theneuralstemcellsfromhumanembryonicbrainsweresuccessfullyculturedandformedtypicalneurospheresinsuspension,andmostofthecellsexpressedvimentin,whichwasamarkerforneuralprogenitorcells,andthecellscoulddifferentiateintoneurons,astrocytesandoligodendrocytes.Invitromyelinformationinneurosphereswereobservedatanearlystageofculture.Conclusions:Humanneuralstemcellscanbeculturedfromembryonicbrains,canformthetypicalneurospheresinsuspensioninvitroandhavetheabilityofmyelinating,andmaybepotentialsourcefortransplantationintreatingmyelindisorders.

简介：Eosinophiliculceroforalmucosa(EUOM)isanonspecificclinicalappearance,whichappearstobefrequentlyunrecognizedormisdiagnosedusuallyassquamouscellcarcinoma(SCC),especiallyinoldpatients.Itisarareself-limitedbenignulcerativedisease,anditischaracterizedwiththeinfiltrationofeosinophilicleukocytesmixedwithotherinflammatorycells,1,2suchasthemitoticallyactivelargemononuclearcellswithroundorovoidpalenucleus.Thiscelltype,ofteninterpretedasahistiocyte,confoundsthehistologicevaluationandoccasionallyleadstotheerroneousdiagnosisoflymphohistiocyticmalignantcondition.Althoughithasassumedthattraumaisanimportantetiologicfactor,theexactpathogenesisremainsunclear.3ThemorbidityofthisdiseaseislowandtherearefewChineseliteraturesaboutit,4soitisnecessarytopaymoreattentiontoit.Inthisarticle,3outof5casesofEUOMaremisdiagnosedasSCC,andwewillreporttherecordsinordertoidentifyitfromSCCwell.

简介：Objective:Tostudythecorrelationbetweenbrainedema,elevatedintracranialpressure(ICP)andcellapoptosisintraumaticbraininjury(TBI).Methods:Inthisstudy,totally42rabbitsin7groupswerestudied.Sixoftheanimalswereidentifiedasacontrolgroup,andtheremaining36animalswereequallydividedinto6TBIgroups.TBImodelswereproducedbythemodifiedmethodofFeeney.Aftertheimpact,ICPofeachsubjectwasrecordedcontinuouslybyanICPmonitoruntiltheanimalwassacrificedatscheduledtime.Theapoptoticbraincellsweredetectedbyanterminaldeoxynucleotide-transferase-mediateddUTP-digoxigeninnickendlabeling(TUNEL)assay.Cerebralwatercontent(CWC)wasmeasuredwithadryingmethodandcalculatedaccordingtotheElliottformula.Then,ananalysiswasconductedtodeterminethecorrelationbetweenthecountofapoptoticcellsandtheclinicalpathologicalchangesofthebrain.Results:Apoptoticcellcountbegantoincrease2haftertheimpact,andreacheditsmaximumabout3daysaftertheimpact.ThepeakvalueofCWCandICPappeared1dayand3daysaftertheimpact,respectively.ApoptoticcellcounthadapositivecorrelationwithCWCandICP.Conclusions:InTBI,occurrenceofbrainedemaandICPincreasemightleadtoapoptosisofbraincells.Anytherapywhichcanrelievebrainedemaand/ordecreaseICPwouldbeabletoreduceneuronapoptosis,therebytoattenuatethesecondarybraindamage.

简介：Objective:　Toexplorethevariantprocessesofcellapoptosisandtheinhibitingeffectofmoderatehypothermiaoncellapoptosisafterdiffusebraininjury.　　Methods:　ModelsofdiffusebraininjurywereinducedbythetraumadevicereportedbyMarmarou.1Atotalof128Wistarratsweredividedinto4groups:theuninjuredgroup(GroupA,n=8),theseverelyinjuredgroup(GroupB,n=60),themildlyinjuredgroup(GroupC,n=30)andthemildhypothermiagroup(GroupD,n=30).InGroupD,theseverelyinjuredratsweretreatedwithmoderatehypothermiatokeeptherectaltemperatureat32℃(standarddeviationfor0.1℃)for6hours.Thenthemorphosis,thecharacteristicsandthequantityofapoptoticcellsinthecerebralcortexandinthehippocampusregionsafterdifferentseveritiesofcraniocerebralinjurieswereobservedandcomparedunderanelectronicmicroscope,withterminaldeoxynucleotidylnickendlabeling(TUNEL)inDNAfragmentationandwithagarosegelelectrophoresis.　　Results:　TUNELshowedapoptoticcellsincreasedaccordingtotheinjuryseverity,andtheypeakedat48hoursafterinjuryandthendeclined.InGroupC,apoptosiswaslocatedintheCA2andCA3areasofthehippocampus.AndinGroupB,apoptosisincreasedevidently,andlocatedinthewholehippocampusandinthefrontalandparietalcortexregions.Thehypothermia-treatedratshadsomeapoptoticcells,too.However,evenat24,48and72hoursafterinjurythereweresignificantlyfewerapoptoticcellsinthecortexandinthehippocampusinGroupDthanthatinthenon-treatedgroups.Electronmicroscopyshowedthattheapoptoticcellswereroundandshrunkeninmorphologyandthenucleiwereroundandcondensedat24and48hoursafterinjury.Andtheapoptosisat48hourswasmoreseverethanthatat24hours.Thehypothermia-treatedratshadnoapoptoticcells.GelelectrophoresisshowedthatcharacteristicDNA“ladders”wereobservedinthecortexandinthehippocampusat48hoursafters

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简介：客观：由经皮的解压缩和自体同源的骨头marrowmononuclear房间(BMC)评估大腿骨的头的处理ofosteonecrosis的临床的功效和安全注入。方法；在有在早舞台的脉管的坏死的28个病人的44个臀部由解压缩由自体同源的BMCsinfusion跟随了的经皮的多重洞被对待。自体同源的BMC从从病人的以后的肠骨的冠被拿的骨头髓被集中。病人们在至少2年上面被跟随。结果被变化在radiograghic阶段在哈里斯臀部分数和前进决定。结果：Nocomplications在操作以后被观察。在操作前，有在8个臀部，在15个臀部的阶段Ⅱ，在14个臀部的阶段Ⅲ，在7个臀部的阶段Ⅳ，和在最近的后续的手术后的阶段的大腿骨的头坏死的阶段Ⅰ是在1个臀部的阶段O，在6个臀部的阶段Ⅰ，在13个臀部的阶段Ⅱ，在13个臀部的阶段Ⅲ，在7个臀部的阶段Ⅳ，在4个臀部的阶段Ⅴ。吝啬的preoperativeHarris臀部分数是58(46-89)，并且改善了到86(70-94)手术后地。外科手术前地折叠的所有大腿骨的头证明坏死的尺寸是至少超过30%。结论:与自体同源的BMC相结合的经皮的多重洞解压缩是对待大腿骨的头的脉管的坏死的一个新方法。越早阶段，越更好结果。Arandomized未来的学习需要以后与平淡的核心解压缩作比较。

简介：客观：为了探索可行性构造遗传工程人，神经干细胞(hNSCs)由lentivirus调停了多表示基因以便为针的绳索损害(SCI)的进一步的研究提供接枝来源。方法：从人的流产胎的大脑外皮的人的神经干细胞被孤立并且有教养，然后，基因被lentivirus修改两个都表示绿荧光蛋白质(GFP)和老鼠neurotrophin-3(NT-3)；转基因的表示被荧光显微镜，胎儿的老鼠的背面的根中心和槽污点的方法检测。结果：遗传工程hNSCs成功地被构造。所有在荧光显微镜下面表示了明亮绿的荧光的遗传工程hNSCs被观察。转基因的hNSCs的调节媒介能导致从背面的根中心(DRG)挥舞长出的神经突。遗传工程hNSCs表示了高级NT-3which能被使用槽污点检测。结论：遗传工程hNSCs调停了bylentivirus能被构造多成功地表示基因。

简介：客观：为了由adipose-derivedstromal房间(ASC)在vitro导致adipocyte区别，与绿荧光灯的蛋白质(GFP)从转基因的老鼠收获了并且估计经由ASC的附件构造脂肪质的纸巾打字的可能性我骨胶原scaffolds.Methods：从GFP转基因的老鼠的腹股沟的胖垫被酶为ASC(主要文化)的隔离消化。在到ASC的三个段落的扩大以后，房间被ASC在vitro在为二个星期，和adipocyte区别中等的anadipogenic孵化被词法观察和油红估计染色的O。然后，他们被纳入为12个小时andco有教养的骨胶原脚手架，为2months由皮下注射的培植列在后面到裸体老鼠的背面的皮肤。最新形成的纸巾被检测由他染色。结果：有教养的主要干细胞是像成纤维细胞、显示出的活跃增长。在在anadipocyte区别媒介被孵化以后，在逐渐地并且最后积累的细胞质的类脂化合物微滴发展成成熟adipocytes，它出现了在染色的油红O积极。0。5厘米的~3new组织块在裸体老鼠的背面的皮下面被发现，它被荧光灯的观察作为成熟脂肪质的纸巾证实并且他染色。结论：ASC能成功地区分脂肪质的纸巾进成熟adipocytes，它作为intracytoplasmic类脂化合物微滴展出象adipocyte一样形态学和快车。它是与ASC和类型设计的脂肪质的纸巾的一个有效模特儿我骨胶原脚手架。

简介：ObjectToexploretileeffectoflipofectin-c-erbB2antisenseoligodeoxynucleotidesonradiosensitivityofhumanovariancancercellllne.MethodsTheexpressionofc-erbB2wasdetectedbymeansofRT-PCR,cellularresponsetoirradiationwasevaluatedbytilecolonyformingassay.ResultsLipofectin-c-erbB2antisenseoligodeoxynucleotides（AS-ODN)couldsuppresstheexpressionofc-erbB2,andsignificantlydecreasedthecolonyformingrateofhumanovariancancercellsafterionizingirradiation（P<0.01),whilenon-trean~entandthesenseoligodeoxynucleotides{S-ODN)groupsdidnotdecreaseontheradio-resistancelevelofSKOV3cellline{P>0.05).Condusionc-erbB2antisenseoligodeoxynueleotidessensitizedtheSKOV3toionizingirradiationthroughdecreasingtheexpressionofe-erbB2,whichmightbetheresultofthefactthatc-erbB2antisenseoligodeoxynueleotidesinhibittheeelluarsignaltransductionpathwayrelatingtotheradiation-resistantphenotype.

简介：目的探讨CELL-DYN1700血细胞分析仪白细胞分类状况。方法随机挑选270份血液标本用仪器法和手工法作白细胞分类对照。结果仪器法与手工法有87．4％的标本是相符的，P〉0．05，还有12．6％的标本与手工法分类有极显著性差异，P〈0．01。结论不能完全依赖仪器分类，结合白细胞分类的报警提示和直方图可在工作时间和人员有限的情况下，准确挑出需要复查的标本，进行手工分类复核，可以大大提高检验质量和效率。