简介：IntroductionRecently,bonemarrowmesenchymalstemcells(MSCs)havebeenreportedtorepairchronicallyinfractedmyocardiumwithdirectinjection.However,itisverydifficulttolocalizetheinjectedcellsontotheischemicareatoregeneratesufficientcardiacmassinthethinnedscararea.Toovercometheproblem,wehaveutilizedourcellsheettechnologybasedontemperature-responsiveculturedishes.Whentheculturetemperatureisreducedfrom37℃to20℃,allcellsconnectedviacell-celljunctionproteinsareharvestedasasinglesheetwithoutusingproteolyticenzymes.Thistechnologyallowsustotransplantstemcellsinvivofortreatmentheartdiseasewithouttheproblemsmentionintheprevious.MethodsMaleClawnminipigswereusedinthisstudy.Bonemarrow(5-7mL)wascollectedundergeneralanesthesia.Histopaqe-1077(15mL),wereaddedtobonemarrowandcentrifuged.Thecellswerecollectedandculturedfor7days.Weseededthebonemarrow-derivedMSCsattheconcentrationof(6×10~5/ml)on60mmdiametertemperature-responsivedishesfor7days.Astheculturetemperaturedecreasedfrom37℃to20℃,MSCsheetdetacheditselfspon-taneouslyandfloatedupintotheculturemedium.Triplelayerswerestackedtogetherrepeatedlyformingspecialmultiplayer.Myocardialinfarctionwascreatedbytheligationoftheleftanteriordescendingbranchoftheleftcoronaryartery.Acellsheetswastransplantedontotheischemiaarea.Theechocardiographywasperformedtwoandfourweeksaftertransplantation.Thehearttissuewithcellsheetswereremovedandfixedwith10%formalinforhistologicalanalysisonemonthafterthetransplantationofcellsheets.ResultsMostMSCsarepositiveforCD29,CD90,CD146andCD73.ThesemeantheculturecellsheetswerecomposedofundifferentiatedMSCsandremainedmultipotent.Monolayers(20-30μm)andmultilayer(120μm)cellsheetswereproduced,whichretainedallcell-to-cellcontaction.Histologicalanalysesshowthecellsheetsbecomecloselycontactedwiththehearttiss

简介：Inthepresentpaper,theauthorputsforwardsixkeypointsforacupuncturetreatmentofdiseases,namely,①carefulexamination,②definitediagnosis,③preciseandappropriateidentificationofsyndromes,④accuratelocationoftheacupoint,⑤flexibleapplicationofneedlingmanipulations,and⑥“Deqi”.Thefirstthreeaspactsarethefoundation,accuratelocationandflexibleneedlingmanipulationsarealsotheprerequisiteforeffectivetreatmentofdiseases.Inaddition,soundtheoreticalbasicknowledgeofbothtraditionalChinesemedicine(TOM)andmodemmedicine,andflexiblyapplyingsuitableneedlingmaneuvers,stimulatingquantityanddurationofneedleretaininginaccordancewiththeconcretestateofdiseaseandthepatient'sconditionsarealsoveryimportantinclinicalpracticeofacupuncture.

简介：Thesurvivalorientedadaptationofevolvedbiosystemstovariationsintheirenvironmentisaselectiveoptimizationprocess.Recognizingtheoptimisedendproductanditsfunctionalityistheclassicalarenaofbionicengineering.Inaprimordialworld,however,themolecularorganizationandfunctionsofprebioticsystemsweresolelydefinedbyformativeprocessesintheirphysicalandchemicalenvironment,forinstance,theinterplaybetweeninterfacialwaterlayersonsurfacesandsolarlight.Theformativepotentialoftheinterplaybetweenlight(laserlight)andinterfacialwaterlayersonsurfaceswasrecentlyexploitedintheformationofsupercubanecarbonnanocrystals.Inevolvedbiosystemstheformativepotentialofinterfacialwaterlayerscanstillbeactivatedbylight.Herewereportacaseofhayfever,whichwassuccessfullytreatedinthecourseofafacialreju-venationprogramstartinginNovember2007.Targetingprimarilyinterfacialwaterlayersonelastinfibresinthewrinkledareas,wepresumablyalsoactivatedmastcellsinthenasalmucosa,reportedtoprogressivelydecreaseinthenasalmucosaoftherabbit,whenfrequentlyirradiated.Hayfeverisinducedbythereleaseofmediators,especiallyhistamine,aprocessassociatedwiththedegranulationofmastcells.Decreaseinmastcellsnumbersimpliesadecreaseinthereleaseofhistamine.Tothebestofourknowledgethisisthefirstreportonthetreatmentofhayfeverwithvisiblelight.Thisapproachwasinspiredbybionicthinking,andcouldhelpamelioratingtheconditionofmillionsofpeoplesufferingfromhayfeverworldwide.

简介：Efficacyofacupuncturetherapyvariesinpatientswithsimilarailments.Thepresentstudywasundertakentosearchforamarkerforbetterefficacyofacupuncturetherapy.Thestudywasmadein224patientsincludingosteoarthritis141(62.94%),polyarthritis23(10.26%),Bursitis&synovitis15(6.69%)andothers45(20.08%).ABObloodgroupsweretestedforeachpatient.ItappearsthatpatientsbelongingtogroupABandBrespondedwelltoacupuncturetherapyinproportionatelymorenumber.Goodresultwasachievedin47.82%casesingroupABand46.04%casesingroupB,whereaspatientsofgroupAandOshowedgoodresultin27.65%and26.15%casesrespectively.ApparentlyitmaybeconcludedthatpatientsofAB&Bbloodgroupswouldrespondcomparativelywelltoacupuncturetherapy.

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简介：关键词艾灸治疗-健康提升艾灸-预防的药鍏抽敭璇？鐏告硶-淇濆仴鐏?-棰勯槻鍖诲CLC数字R245.8资金项目：中国的国家基本研究节目(973编程序，2009CB522904)；湖南省(08C633)作者的教育部门的工程：YANJie(1941鈥?，女性，教授，博士优异通讯作者：吴焕干(1956鈥?，男性，教授，博士上级

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简介：AbstractGlioblastoma (GBM) is the most common primary malignancy of the central nervous system in adults. The prognosis for late-stage glioblastoma (World Health Organization grade IV astrocytic glioma) is very poor. Novel treatment options are sought after and evaluated by clinicians and researchers, and remarkable advances have been made in surgical techniques, radiotherapy, and chemotherapy. However, the treatment of glioblastoma remains extremely difficult and it can extend the lives of patients by only a few months. There has been notable progress in the field of immunotherapy, particularly with the use of tumor vaccines, for treating glioblastoma; especially peptide vaccines and cell-based vaccines such as dendritic cell vaccines and tumor cell vaccines. However, the results of the current clinical trials for vaccination are not satisfactory. This article reviews the progress in the development of vaccines for glioblastoma.

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简介：Over20yearsofresearchhasledtothenowwidelyacceptedroleofcardiacresynchronizationtherapy(CRT)inmedicallyrefractory,mildtoseveresystolicheartfailure(HF)withincreasedQRSduration.Inadditiontoconferringamortalitybenefit,CRThasbeenshowntoreduceHFhospitalizationratesandimprovefunctionalstatusinthispopulation.However,notallpatientsconsistentlydemonstrateapositiveresponsetoCRT.EffortstoimproveresponsetoCRThavefocusedonimprovingpatientselectionandoptimizingdeviceimplantationandfollow-up,therebycorrectingelectricalandmechanicaldyssynchrony.Inthisarticlewereviewthepathobiologyofcardiacdyssynchrony,therationalefortheuseofCRT,thehistoryandthestateoftheartofCRT,andguidelinesandrecommendationsforCRT,whilealsofocusingontheareasofcontroversyandpotentialfutureapplications.

简介：目的：观察推拿手法治疗颈源性头痛的临床疗效。方法：54例患者随机分为推拿组和药物组，推拿组27例，接受中医推拿治疗，药物组27例，口服布洛芬治疗。比较治疗前2星期和治疗后2星期病患的头痛程度（VAS）、头痛发作频率和颈椎功能障碍（NDI）的改善。结果：治疗前推拿组和药物组的VAS，发作频率和NDI评分差异无统计学意义；治疗后两组VAS，发作频率，NDI均有下降，推拿组与药组比较差异有统计学意义（P〈0．01）。结论：推拿治疗颈源性头痛疗效优于常规剂量布洛芬。

简介：Dermatofibrosarcomaprotuberans(DFSP),themostcommondermalsarcoma,isalow-grade,slowgrowingfibroblasticmalignantneoplasmthatmostfrequentlyaffectsmiddleagedadultsandischaracterizedbyahighlocalrecurrencerateandalowpropensityformetastasis.WidesurgicalresectionorMohsmicrographicsurgery(MMS)arethepreferredapproachesforlocalizeddisease,whileradiationtherapyiswarrantedforinoperablediseaseorforcaseswithpositivemarginswherere-excisionisnotpossible.DFSPisgenerallyregardedasrefractorytoconventionalchemotherapy.Treatmentoptionsforsystemicdiseasewerelimiteduntilthediscoveryofauniquetranslocation,t(17;22)(q22;q13)(COL1A1;PDGFB)foundinamajorityofcases.Inrecentyears,imatinib,aPDGFβR,ABLandKITinhibitor,hasrevolutionizedsystemictherapyinDFSP.Inthisreview,wesummarizetheepidemiological,clinical,histologicalandgeneticcharacteristicsofDFSPandupdatethereadersonitscurrentmanagement.

简介：Thedeveloppercutaneoustreatmentsformitralregurgitation(MR)havebeenbasedonestablishedsurgicalprocedures.Mostarebasedinsomewayonmitralannuloplasty.IndirectangioplastyutilizingcoronarysinusandleafletrepairwiththeMitraClipdevicehavethemostdevelopmentandclinicalapplication.Morerecently,afterthesuccessoftranscatheteraorticvalvereplacement,transcathetermitralvalvereplacementhasemerged.Acriticalunansweredquestioniswhattherelativerolesofvalverepairandvalvereplacementwillbe.ThelargestexperienceinpracticeiswithMitraClipleafletrepair.Theevidencebaseforthedevelopmentofthesenoveltherapiesincludessomedatainsurgicalcandidates,andregistrystudiesthathavebeendonepredominantlyinhighriskpopulations.

简介：WhenIcameacrossLamaGangcheninNepalyearsago,hewasthenresidinginItaly.HehasagoodcommandofBuddhistTantrictheoryandpractice,aswellaswideknowledgeofTibetanmedicine.HeisalsoactiveinpropagatingTibetanBuddhismandTibetanmedicine,especiallyinworkingtoprotectthepsychicenvironmentandpromoteeducationandcharityundertakings.Forthispurpose,hehasvisitedmorethan30countriesandregions.

简介：Ameloblastomaisabenignbutlocallyaggressiveodontogenieneoplasmthataccountsfor10%ofalltumorsarisinginthemandibleandmaxilla(1).Eightypercentofameloblastomasariseinthemandible,andtheyareusuallyfoundinyoungadults.Itfrequentlyrecursifnotadequatelyresected.Therefore,thestandardtherapyforthistumoriscompleteboneresectionwithanadequatemarginofsafety:marginalorsegmentalosteotomy.However,aestheticdeformities,functionalimpairmentsandpsychologicalimpairmentsafterradicalsurgeryforlargeameloblastoma,havebeenseriousissues(1).

简介：Translationalresearch,ingeneral,meanstotakeknowledgefromoneareaintoasecond.Traditionalthoughttorepresenttransferofknowledgefrombasicresearchintotheclinic,translationalresearchtodaycoversamuchbroaderterm.Thus,mostinvestigatorswillagreetranslationalresearchcoversatwo-wayprocessthatalsoincludestakinglessonsfromtheclinicbacktothelaboratory,bywhichbiologicalobservationsinvivowouldcreatenovelhypothesestobefurtherexploredinlaboratoryexperiments.

简介：130例单纯性肥胖症患者经针刺和耳穴贴压治疗，经1个疗程治疗后，显效49例，有效73例，无效8例，总有效率为93．8％。尤其以重度肥胖症的减肥效果最佳。

简介：Anticancerimmunotherapyhasundergonealongevolvingjourneyfordecades,andhasbeendramaticallyappliedtomainstreamtreatmentsinoncologyinrecent5years.Thisprogressrepresentsanadvancedmilestonefollowingcytotoxicmedicineandtargetedtherapy.Cellularimmunityplaysapivotalroleintheimmuneresponsesofhoststotumorantigens.Suchimmunityisnotablysuppressedduringneoplasticprogressionduetoimmuno-editingprocesses.Cellularimmunitycanalsobeselectivelyreactivatedtocombatmalignancieswhileexploitingtheadvantagesofcontemporaryscientificbreakthroughsinmolecularimmunologyandgeneticengineering.Therapidadvancementofcellularimmunity-basedtherapeuticapproacheshasachievedhighefficacyincertaincancerpatients.Consequently,thelandscapeofoncologicmedicineandpharmaceuticalinnovationhastransformedrecently.Inthisregard,wepresentacomprehensiveupdateonclinicallyestablishedanti-cancertreatmentswithcellimmunityaugmentationasthemajormechanismofaction.

简介：以简易埋针法治疗呃逆。取膈俞穴耳穴耳中(膈)和胃埋针，治疗呃逆症患者24例。结果症状消失21例，缓解1例，无效2例。部分患者的呃逆症状可即刻停止发作。该法收效快速，维持疗效时间长．

简介：Photothermalcancertherapyisanalternativetochemotherapy,radiotherapy,andsurgery.Withthedevelopmentofnanophotothermalagents,thistherapyholdsimmensepotentialinclinicaltranslation.However,thetoxicityissuesderivedfromthefactthatnanomaterialsaretrappedandretainedinthereticuloendothelialsystemslimittheirbiomedicalapplication.Developingbiodegradablephotothermalagentsisthemostpracticalroutetoaddresstheseconcerns.Inadditiontothephysicochemicalpropertiesofnanomaterials,variousinternalandexternalstimuliplaykeyrolesonnanomaterialsuptake,transport,andclearance.Inthisreview,wesummarizednovelnanoplatformsforphotothermaltherapy;thesenanoplatformscanelicitstimuli-triggereddegradation.Wefocusedontherecentinnovativedesignsendowedwithbiodegradablephotothermalagentsunderdifferentstimuli,includingenzyme,pH,andnear-infrared(NIR)laser.