简介：Inthepresentstudy,theeffectofblockageofthecostimulatorysignalCD86attimeofimplantationontheexpressionsofTGF-β1,MMP-9,TIMP-3andPAI-1proteinsatthematernal-fetalinterfaceandtheoutcomeofpregnancyinmurineabortion-pronemodelwasinvestigated,inwhichtheCBA/JxDBA/2matingswereusedastheabortion-pronemodelandtheCBA/J×BALB/cmatingsusedasthenormalpregnantmodel.Thestudywasperformedinfollowingthreegroups:2groupsoftheabortion-pronemodel,whichwereexperimentalgroupandcontrolexperimentalgroup,and1groupofnormalpregnantmodel,andeachgrouphad10pregnantCBA/Jmiceexclusively.FemalepregnantCBA/Jmiceintheexperimentalgroupreceivedanintraperitoneal(i.p.)injectionof100μgofantimouseCD86mAbin200μlofPBSatday4.5ofgestation,andtheirrelevant-isotopematchedratIgG2bwasadministratedinthecontrolexperimentalgroupwiththesamedosageandatsametime.Forthenormalpregnantgroup,notreatmentwasgiven.ThepregnantCBA/Jmicewerekilledonday13.5ofgestation.Then,theembryoresorptionratewascalculatedandtheexpressionsofTGF-β1,MMP-9,TIMP3andPAI-1weredetectedbyusingimmunohistochemicalmethods.Itwasdemonstratedthattheembryoresorptionrateintheexperimentalgroupwassignificantlyreducedincomparisonwiththatinthecontrolexperimentalgroup(x2=7.441,P=0.006),buttherewasnosignificantdifferencewiththatinnormalpregnantgroup(x2=0.016,P=0.898).TheexpressionsofTGF-β1andPAI-1intheexperimentalgroupweresignificantlyincreasedincomparisonwiththatinthecontrolexperimentalgroup(P=0.010,P=0.003,respectively),withnosignificantdifferencefromthatinthenormalpregnantgroup(P=0.500).However,theexpressionofMMP-9intheexperimentalgroupwassignificantlyreducedincomparisonwiththatinthecontrolexperimentalgroup(P=0.012)withnosignificantdifferencefromthatinthenormalpregnantgroup(P=0.500).Theexpression

简介：ExposureofnaivemurineCD4^+TlymphocytestosuperantigensuchasstaphylococcalenterotoxinB(SEB)inducesastrongproliferativeresponse.ProlongedexposureorsubsequentrestimulationoftherespondingTcellpopulationwithSEBleadstotheapoptoticeventsofactivation-inducedcelldeath(AICD).ThesignalingmechanismresponsiblefortheAICDisatargetofintensiveinvestigation.However,theprecisedownstreamsignahngpathwaysofSEB-inducedAICDremainsunclear.Ourresultshereshowthatthesequentialactivationofcaspase-1/ICE-hkeandcaspase-3/CPP32-hkecysteineproteasesprobablyplaysaroleinthesignalingtransductionofSEB-inducedAICD,butcaspase-3/CPP32-hkeproteasesactivationdoesnotdependoncaspase-1-likeproteasesactivation.HerbimycinA,aspecificinhibitorofproteintyresinekinases,inhibitcaspase-3/CPP32-1ikecysteineproteasesactivation.However,itdoesnotpreventDNAfragmentationofCD4^+TcellsapoptosisinducedbySEB.TheseresultsindicatethatproteintyrosinekinasespathwayisprobablyinvolvedinthesignalingtransductionofCD4^+TcellsapoptosisinducedbySEBand“crosstalks”withthepathwayofcaspase-3/CPP32-1ikeproteasesactivation.

简介：摘要：目的：探讨于3D全腹腔镜下胰十二脂肠切除术患者行手术室标准化护理的作用。方法：本研究按照患者的入院时间进行分割，收集60例行3D全腹腔镜下胰十二脂肠切除术患者作为客观研究对象，利用数据奇偶排列方式，将其中30例患者设立为实验组，剩余30例均为对照组，两组患者统一实施3D全腹腔镜下胰十二脂肠切除术，对照组患者在这一过程中实施基本护理模式，实验组患者在原有护理模式基础上予以手术室标准化护理，对比两组患者的干预效果维度变化以及护理满意维度变化。结果：不同护理模式实施后，实验组的各个维度指标，包括护理态度、质量以及人文关怀等维度均有明显上升，两组对比存在差异较大，组间差存意义，（P

简介：【摘要】目的：探讨环磷酰胺联合小剂量激素治疗膜性肾病的疗效及血清PLA2R、25（OH）D3、NGAL水平。方法：选取我院2018年3月-2020年5月时期内收治的膜性肾病患者共80例，以抽签法纳入A组（40例）和B组（40例）。A组使用环磷酰胺治疗，B组使用环磷酰胺联合小剂量激素治疗，比较患者总有效率、血清PLA2R、25（OH）D3、NGAL水平。结果：和A组相比，B组总有效率较高，血清PLA2R、NGAL水平较低，25（OH）D3水平较高，数据比较有意义（P＜0.05）。结论：针对膜性肾病患者，环磷酰胺联合小剂量激素治疗模式，不仅可提高患者整体治疗效果，还可改善血清水平，可推广。