简介：维生素D3起来调整的蛋白质(VDUP1)1是涉及维持细胞的动态平衡的多功能的蛋白质。VDUP1被许多压力导致。相反地，VDUP1经常在各种各样的肿瘤纸巾和房间线被减少。VDUP1的在表示上通过房间周期拘捕禁止房间增长。VDUP1与thioredoxin(Trx)交往并且否定地调整表示和涉及氧化还原作用规定的Trx的抗氧化剂功能。VDUP1-/-鼠标比野类型的鼠标更产生carcinogenesis并且在包括自然漂亮房间的开发和函数建立免疫系统是有缺点的。而且，VDUP1-/-mice表演损害了Kreb调停周期的丰满的酸利用。在这评论，我们在多样的细胞的回答，特别地与增长的它的关系，apoptosis，区别，和象癌症那样的疾病和压力相关的疾病讨论了VDUP1的多功能的角色。

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简介：Aim:Toinvestigateglycoconjugatechangesonthecellsurfaceofproliferativelesionsandneoplasmsofmicelungsatvariousstagesoftumorigenesis,therelationbetweenprogressivedevelopmentofmousepulmonarytumorsandexpressionofcellsurfacesaccharide.Materialsandmethods:Thirty-onemaleA/Jstrainmiceat5weeksofageweretreatedintraperitoneallywithasingleinjectionof20-methylcholanthrene(20-MC),292pulmonarylesionsincluding31hyperplasias,145alveolaradenomas,61papillaryadenomas,55papillaryadenocarcinomasandtheircombinedtypewereobtained.Thebindingaffinitiesofcellsinnormalrespiratoryepitheliaandinproliferativelesionstofourperoxidases-conjugatedlectins,Maclurapomiferaagglutinin(MPA),Arachishypogeaagglutinin(PNA),Ricinuscommunisagglutinin(RCA),andwheatgermagglutinin(WGA)wereexamined.Results:Cellsofhyperplasiaandalveolaradenomashowedfairlystrongaffinitytoallthefourlectins.However,partofpapillaryadenomacellsandgreaterpartofpapillaryadenocarcinomacellslosttheirbindingaffinitytoMPA,PNA,andRCA,butnottoWGA.ThebindingsofMPA,PNAandRNAweredetectedpredominentlyontheluminalsurfacesofbenigntumorsbutnotontheluminalsurfacesofmalignanttumors.WGAmightbindtovariedtypesofbenignandmalignanttumors.Pretreatedwithneuraminidase,thelesionsenhancedthestainingintensityforthefourlectins,thebindingsitesofWGAtomalignanttumorcellswerenumerous.Adistinctdifferenceinlectinbindingaffinitybetweenhyperplasia/alveolaradenoma/papillaryadenomaandpapillaryadenocarcinomawasclearlyshown(x2=46.89,P＜0.01,x2=36.77,P＜0.01andx2=52.87,P＜0.01)inthisexperiment.Thecomplexglycoconjugatesonthecellsurfaceofmalignantandbenignlesionsduringthedevelopmentofpulmonarytumorwerechanged,malignanttumorcellsdifferedfromthesurfaceofbenigntumorcells,thelevelsoftotalsialicacidwerehigherinmalignanttumorce

简介：披风房间淋巴瘤(MCL)是B房间non-Hodgkin淋巴瘤的好攻击的histotype。疾病不知道了痊愈，它为新奇治疗学的代理人推动迫切需要。(CRM1)Chromosomal区域维护1可以在人的瘤形成起一个作用并且用作癌症治疗的一个新奇目标。这研究总结MCL致病并且在贡献MCL致病的几条重要发信号小径的规定决定CRM1的参与，包括房间周期前进，DNA损坏反应，phosphoinositidekinase-3，原子factor-B激活，和chromosomal稳定性的小径。现出症状之前的潜的研究也被介绍在vitro并且在vivo在MCL在MCL房间线和主要MCL房间把CRM1地位与正常B房间，以及CRM1抑制的治疗学的效率作比较，它使这些代理人成为新奇MCL治疗的潜在的目标。

简介：Ras-associatedprotein-1(Rap1),asmallGTPaseintheRas-relatedproteinfamily,isanimportantregulatorofbasiccellularfunctions(e.g.,formationandcontrolofcelladhesionsandjunctions),cellularmigration,andpolarization.Throughitsinteractionwithotherproteins,Rap1playsmanyrolesduringcellinvasionandmetastasisindifferentcancers.ThebasicfunctionofRap1isstraightforward;itactsasaswitchduringcellularsignalingtransductionandregulatedbyitsbindingtoeitherguanosinetriphosphate(GTP)orguanosinediphosphate(GDP).However,itsremarkablydiversefunctionisrenderedbyitsinterplaywithalargenumberofdistinctRapguaninenucleotideexchangefactorsandRapGTPaseactivatingproteins.ThisreviewsummarizesthemechanismsbywhichRap1signalingcanregulatecellinvasionandmetastasis,focusingonitsrolesinintegrinandcadherinregulation,RhoGTPasecontrol,andmatrixmetalloproteinaseexpression.

简介：AbstractWith the deepening of research, proteomics has developed into a science covering the study of all the structural and functional characteristics of proteins and the dynamic change rules. The essence of various biological activities is revealed from the perspectives of the biological structure, functional activity and corresponding regulatory mechanism of proteins by proteomics. Among them, phospholipid-binding protein is one of the hotspots of proteomics, especially annexin A1, which is widely present in various tissues and cells of the body. It has the capability of binding to phospholipid membranes reversibly in a calcium ion dependent manner. In order to provide possible research ideas for researchers, who are interested in this protein, the biological effects of annexin A1, such as inflammatory regulation, cell signal transduction, cell proliferation, differentiation and apoptosis are described in this paper.

简介：Objective:Tostudytheexpressionofvascularendothelialgrowthfactor(VEGF)andmicrovesseldensity(MVD)inesophagealsquamouscellcarcinoma(ESCC)andclarifytheassociationofVEGFexpressionwiththeangiogenesisandprognosticvalueofthisdisease.Methods:Eighty-twocaseswithprimaryESCCtreatedwithradicaloperationinDepartmentofSurgeryfromJan1981throughMay1994wereenrolled.VEGFexpressionandMVDvaluewereexaminedbyimmunohistochemicalstaining,thestreptavidin-biotinperoxidasecomplexmethod(SPmethod),usinganti-VEGFpolyclonalantibodyandanti-Factor-VIIIantibody,respectively.WealsoanalyzedtherelationshipbetweenVEGFexpressionandMVDvalueandpostoperativesurvivalrateofpatients.Results:Ofthe82cases,63.4%casesshowedpositiveforVEGFintumorcellsandthemedianofMVDintumorwas37(9-150)·mm-2.TherewasaclosecorrelationbetweenMVDandVEGF(P=0.001).The5-yearsurvivalrateofpatientswithlowandhighMVDwas34.1%and12.2%,respectively.The5-yearsurvivalratewas46.7%inpatientswithVEGF-negativetumorand11.5%inpatientswithVEGF-positivetumor.Thesedifferenceswerestatisticallysignificant(P=0.017andP<0.001,respectively).Conclusion:InESCC,angiogenesisismediatedmainlybyVEGFandVEGFmaybeassociatedwithtumorprogressionandincreasedmalignancyviaangiogenesis.

简介：Aim:Toisolateandtransplantgermcellsfromadultmousetestesfortransplantation.Methods:Inordertodistinguishtransplantedcellsfromendogenouscellsofrecipients,donortransgenicmiceexpressinggreenfluorescentprotein(GFP)wereused.Germcellswerecollectedfromthedonorsat10-12weeksofageandspermatogoniawereconcentratedbypercollfractionationandtransplantedintorecipientseminiferoustubulesthathadbeenpreviouslytreatedwithbusulfanat5weeksofagetoremovetheendogenousspermatogeniccells.Results:Twentyweeksafterthetransplantation,awidespreadGFPsignalwasobservedintherecipientseminiferoustubules.Thepresenceofspermatogenesisandspermatozoawasconfirmedinsectionsof12outof14testestransplanted(86%).However,whengermcellsweretransplantedwithoutconcentrationthesuccessratewaszero(0/9).Conclusion:Germcellsfromadultmousetestescanbesuccessfullytransplantedintorecipientseminiferoustubulesifthecellpopulationisrichinspermatogoniaandthepercollfractionationisusefulinobtainingsuchacellpopulation.

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简介：Theresearchgroupwasinterestedinthetherapeuticpotentialoflimbalfibroblasts,whicharepre-sentinthecorneallimbus.Recently,inmuchstemcellresearch,researchersandadvocatesarestress-ingtheimportanceofutilizingcellsfromthenichewhichtheywilltarget.Thismeans,thatadiposetissueandbonemarrowderivedstemcells,arelikelynotappropriateandevendangeroustoapplyu-biquitouslyastherapiestootherpartsofthebodyincertaincases.Severalresearchgroupsandclini-caltrialshaveshownthistobethecase,includingcasesofblindness,braininflammation,anddeathasadverseevents.

简介：Objective:Langerhanscellhistiocytosis(LCH)hasbeenwelldescribedonlyinchildren.Weanalyzedthecharacteristics,reactivation,andoutcomeofLCHinacohortof55patientsacrossallages.Methods:WereviewedtherecordsofallpatientswithLCHtreatedatasingleinstitutebetweenJan.1974andMay1998.Results:The55patientswere2to67yearsofage(median,31years)atthetimeofdiagnosis,and85.5%weremale.Fortypatients(72.7%)hadsingle-systemLCH;Fifteen(27.3%)hadmultisystemdisease.Theheadandneckwasthemostfrequenttumorsite(63.6%).LCHwasnotfoundinorgansatriskofinvolvement(liver,spleen,bonemarrow,andlungs).Thefrequencyofbonyinvasion(23.6%overall)differedsignificantlyaccordingtoage(15years(66.7%)vs.Age>15years(11.6%)(P=0.0005).Atamedianfollow-upof12years,nopatientdiedofLCH.The5,10-yearsurvivalestimateswere100%.The5,10-yeardisease-freesurvivalestimateswere70.9%and58.4%.The5-yeardisease-freesurvivalestimatewas58.3%forage(15yearsvs.74.4%forage>15years(P=0.83)and75%forsingle-systemdiseasevs.60%formultisystemdisease(P=0.13).LCHwasreactivatedin43.6%ofpatients,withamedianof14months(range,2-180months).Threepatientswithrecurrentdiseaseexperiencedspontaneousremission.Atthetimeofthemostrecentfollow-up,23.6%ofsurvivorshadactivedisease.Conclusion:LCHisnotfoundexclusivelyinchildrenandadolescents.Thefrequencyofboneinvasionisinverselyrelatedtoage.Reactivationisverycommonregardlessofthetypeoftreatment,buttheprognosisisgenerallygood.

简介：TheDNAcontentandmorphometricfeaturesofhepatocellularcarcinoma(HCC)andlivercelldysplasia(LCD),includingnucleararea,nuclearperimeter,nuclearmaximumdiameterandnuclearcirclediameter,werequantitativelydeterminedbymeansofimageanalysistechnology.Theresultsshowedthatincomparisonwithnormalhepatocytes,LCDhadamarkedlyincreasedDNAcontentandnuclearmorphometricparameters,butthevalueswerelowerthanthoseforHCC.LCDshowedaslightincreaseinnuclearatypiarepresentedbythenuclearirregularindex,whichwasalsolessthanHCC.ThefindingsindicatethatLCDmaybeaprecaneerouslesionofHCC,tothecellsinanabnormalproliferativestate.

简介：AbstractIntroduction:There is a known association between primary mediastinal germ cell tumor (PMGCT) and hematologic malignancy that is not linked to treatment. They are exceptionally rare entities with a low morbidity and a poor prognosis.Case presentation:An 11-year-old boy presented with an anterior mediastinal mass diagnosed as a malignant germ cell tumor on the basis of an excisional biopsy. He was found to have acute myeloid leukemia (AML) two years after the chemotherapy for his germ cell tumor. The clinical course was very aggressive with a survival time of only 1 week after diagnosis of AML associated with PMGCT.Conclusion:AML associated with PMGCT needs to be diagnosed correctly. Relevant examinations should be carried out in patients with PMGCTs during and after chemotherapy, and long-term follow-up is still necessary to reduce the risk of morbidity and mortality.

简介：CD8+T房间在对感染向保护提供细胞内部的病原体和一些肿瘤起一个枢轴的作用，这很好被接受。在许多情况中，保护的免疫为时间(免疫学的记忆)的长经期被维持。在过去的年，不得不以便完成这些多重任务，受动器的不同子集和记忆T房间被产生，变得明显。然而，直到今天，很少对子集区别并且预定系命运决定的内在的机制被知道。在这上下文，在哪个功能的同种细胞的扩大和phenotypical异质的水平被完成决定具有特殊重要性。为T房间子集多样化的不同模型被建议了；这些在priming和同种细胞的扩大期间主要在时间点不同(优先，在期间，或在第一个房间部门以外)区别什么时候编程序，被导致。最近发达的单个房间的采纳转移技术允许我们证明那个单个先锋房间仍然忍受完整的粘性开发不同T房间子集进过多。这观察指向向在第一个房间部门以外仍然是起作用的因素塑造T房间子集区别。这些调查结果为疫苗的开发有重要含意，，向不同子集的区别模式的调整能成为强大的策略提高疫苗的功效和质量。

简介：Objective:Toclonemultidrugresistance(MDR)relatedgenesinlungadenocarcinomacelllines.Methods:ThedifferentiallyexpressedcDNAfragmentsbetweenA549andA549DDPcellswereanalyzedbymRNAdifferentialdisplayPCR(DDRT-PCR).ThefragmentsthusobtainedwerefurtheranalyzedbyDNAsequencingandNorthernblotting.Results:ThreedifferentiallyexpressedcDNAfragmentswereobtainedandconfirmedbyNorthernblot.Sequenceanalysisrevealedthattwoofthemwerenovelandonewas100%identicalwithICEgene.Conclusion:AnalyzingdifferentiallyexpressedfragmentbetweenA549andA549DDPcellsmaybehelpfulforfindingnewMDRrelatedgenes.ThedrugresistanceofA549DDPcellsmayberelatedtotheinhibitionordown-regulationofICEgene.

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简介：食物过敏症是世界范围的一个主要健康问题。桅杆房间在桅杆房间degranulation为需要广泛地被学习的立即的超敏性起一个很重要的作用。在这研究，一条途径被采取在vitro学习敏化的桅杆房间degranulation的特征，它与桅杆房间和动物模型的学习联系了。BALB/c老鼠被几食物变应原分别地使免疫，然后，血和腹桅杆房间在不同时间点被收集。一颗动态决心在桅杆房间和serumalIgE之间被执行。顺序的时间点上的比较分析证明在敏化的BALB/c老鼠在桅杆房间degranulation和IgE抗体titers之间有靠近的巧合。而且，敏化的桅杆房间能在vitro对挑战实现特定的degranulation，有趣，但是仔细，tropomyosins导致了桅杆房间degranulation显示的生气反应。这很类似于在vivo抵抗变应原的IgE。学习在桅杆房间上揭示了一些特征，来自敏化的BALB/c老鼠，在vitro的degranulation。

简介：瞄准：在食道的有鳞的房间癌(ESCC)调查midkine的表示并且与clinicopathological特征分析它的关系。方法：RT-PCR和免疫细胞化学的染色被用来分别地在EC109房间检测midkinemRNA和蛋白质的表示。然后，在ESCC样品的66种情况中的midkine的表示被免疫组织化学对人的midkine用单音的同种细胞的抗体检测。结果：Midkine被RT-PCR和免疫细胞化学在EC109房间表示。免疫反应在56.1%被检测(37/66)ESCC样品。midkine的表示在肿瘤房间的细胞质被发现。尤其是，midkine的紧张在充满容器和肿瘤的入侵的边阶的区域是更强壮的。Midkine更强烈地比在中等并且糟糕区分的肿瘤在很好区分的肿瘤(76.9%)被表示(43.1%和41.2%，分别地)(P<0.05)。在midkine之间没有统计上重要的关联在ESCC的表示和性，年龄，临床的阶段，淋巴节点转移或幸存。结论：Midkine完了在ESCC表示了。它可以在肿瘤血管生成和侵略起一个作用。midkine的表示在ESCC与肿瘤细胞分化被相关。肿瘤房间区分越多糟糕，midkine表示越多weaker。

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