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简介：ＴＢｕｒｎＲｅｓｅａｒｃｈＩｎｓｔｉｔｕｔｅ，ＳｏｕｔｈｗｅｓｔｅｒｎＨｏｓｐｉｔａｌ，ＴｈｉｒｄＭｉｌｉｔａｒｙＭｅｄｉｃａｌＣｏｌｅｇｅ，Ｃｈｏｎｇｑｉｎｇ４０００３８，Ｃｈｉｎａ（ＣｈｉＬＸ，ＹａｎｇＺＣ，ＷａｎｇＸａｎｄＬｉＡ）Ｔｈｉｓｓｔ...

简介：Cisplatin,awidelyusedanticancerdrug,damageshaircellsincochlearorganotypicculturesatlowdoses,butparadoxicallycauseslittledamageathighdosesresultinginaU-shapeddose-responsefunction.Todetermineifthecisplatindose-responsefunctionforvestibularhaircellsfollowsasimilarpattern,wetreatedvestibularorganotypiccultureswithdosesofcisplatinrangingfrom10to1000μM.Vestibularhaircelllesionsprogressivelyincreasedasthedoseofcisplatinincreasedwithmaximumdamageoccurringaround50–100μM,butthelesionsprogressivelydecreasedathigherdosesresultinginlittlehaircelllossat1000μM.TheU-shapeddoseresponsefunctionforcisplatin-treatedvestibularhaircellsincultureappearstoberegulatedbycoppertransporters,Ctr1,ATP7AandATP7B,thatdose-dependentlyregulatetheuptake,sequestrationandextrusionofcisplatin.

简介：Laser-inducedbreakdownspectroscopy(LIBS)wasexaminedtodetectatracesubstanceadheredontoAlalloysforthesurfaceinspectionofmaterialstobeadhesivelybonded.AsanexampleofSicontamination,siliconeoilwasemployedandsprayedontosubstrateswithacontrolledsurfaceconcentration.LIBSmeasurementsemployingnanosecondUVpulses(λ=266nm)andanoff-axisemissioncollectionsystemwithdifferentdetectingheightswereperformed.Becausesurfacecontaminantsareinvolvedintheplasmaformedbylaserablationofthesubstrates,therelativecontributionofthesurfacecontaminantsandthesubstratestotheplasmaemissioncouldbechangeddependingontheconditionsforplasmaformation.Thelimitofdetection(LOD)wasevaluatedunderseveraldetectingconditionsforinvestigatingthefactorsthataffectedtheLOD.Asignificantfactorwasthestandarddeviationvaluesofsignalintensitiesobtainedforthecleansubstrates.Thisvaluevarieddependingonthemeasurementconditions.FortheAlalloy(A6061),thesmallestLODobtainedwas0.529μg·cm^-2.Furthermore,animprovedLOD(0.299μg·cm^-2)wasobtainedfortheAlalloywithalowerSicontent.

简介：ThepresentstudywasdesignedtodeterminetheeffectsofatraditionalChinesemedicine,calledQishenYiqiDroppingPillonchronichypoxia-inducedmyocardialinjury.ToestablisharatchronichypoxiamodeltobeusedintheevaluationofthetherapeuticeffectsoftheQishenYiqiDroppingPill,Sprague-Dawley(SD)ratswererandomlydividedintothreegroups:thecontrol,model,andtreatmentgroups(n=10pergroup).Theanimalswerehousedinaplexiglasscontainer.Thecontrolanimalswereundernormaloxygenconcentrationandthemodelandtreatmentgroupswereexposedtoairandnitrogenfor5weeks.TheratsinthetreatmentgroupwereorallyadministeredtheQishenYiqiDroppingpill(35mg·kg-1·d-1)for5weeks.Afterthetreatment,thecardiacfunctionandmorphologywereanalyzed,andtheexpressionlevelsofhypoxia-induciblefactor1α(HIF-1α)weredeterminedusingWesternblotting.Ourresultsindicatedthatthecardiacfunctionwasimpaired,cellapoptosiswasenhanced,andHIF-1αexpressionwasup-regulatedinthemodelgroup,comparedtothecontrolgroup.ThesechangeswereamelioratedbythetreatmentwiththeQishenYiqiDroppingPill.Inconclusion,QishenYiqiDroppingpillcanamelioratemyocardialinjuryinducedbychronichypoxia,improvecardiacfunction,anddecreasemyocardialcellapoptosis,whichmayprovideabasisforitsclinicaluseforthetreatmentofchroniccardiovasculardiseases

简介：SeacucumberandcordycepssinensisareusedasbothfoodandtraditionalmedicinesinAsia.Thisstudywascarriedoutinordertoinvestigatethehpyerglycemiceffectofamixtureofseacucumber(Apostichopusjaponicas)andcordycepssinensis(Cordycepsmilitaris)(SCC)indiabeticratandexplorethemechanismunderliningsuchaneffect.Thediabeticmodelratwasinducedwithintraperitonealinjectionofstreptozotocin(STZ).Thediabeticmodelratswererandomlydividedintocontrolgroup(0.9%NaCl),lowdosegroup(300mgSCC·(kgbodyweight)-1)andhighdosegroup(1200mgSCC(kgbodyweight)-1).SodiumchlorideandSCCwereintragastricallyadministeredonceadayfor35d.Changesinfastingserumglucoseandseruminsulincontent,oralglucosetoleranceandliverandmuscleglycogencontentwereroutinelyevaluated.Pancreastissueandβ-cellsofisletswereobservedunderbothopticalandtransmissionelectronicmicroscope,respectively.Theabundanceofglucosemetabolism-relatinggenesingastrocnemiusandepididymaladiposetissuewasdeterminedwitheitherreversetranscriptionPCR(RT-PCR)orwesternblotting.ResultsshowedthatSCCsignificantlydecreasedfastingserumglucosecontent,improvedglucosetoleranceandincreasedseruminsulinandglycogencontent;repairedSTZ-injuredβ-cellsofdiabeticrat,andincreasedtheexpressionofphosphatidylinositol3kinase(PI(3)K),proteinkinaseB(PKB)andglucosetransporter4(Glut4)encodingproteininbothgastrocnemiusandadiposetissue,andGlut4encodinggeneinperipheraltissue.OurfindingsdemonstratedthatSCCexertedananti-hyperglycemiceffectbyrepairingβ-cellsandpromotinginsulin-mediatedsignaltransductionpathwayininsulin-sensitivegastrocnemiusandadiposetissue.

简介：Laser-inducedbreakdownspectroscopy(LIBS)wasdevelopedtodetectaluminuminnickel-basedsuperalloys(K417,GH4033,DZ125L,(?)742y)usinganon-intensified,non-gated,low-costdetectionsystem.TheprecisionofLIBSdependsstronglyontheexperimentalconditions.ThecalibrationcurvesofA1(I)394.4nmandA1(I)396.2nmundertheoptimumexperimentalparametersarepresented.Finallythelimitofdetection(LOD)foraluminumiscalculatedfromtheexperimentaldata,whichisintherangeof0.09%to0.1%byweight.

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简介：Unlikeatraditionalfiberlaserwithadefinedresonantcavity,arandomfiberlaser(RFL),whoseoperationisbasedondistributedfeedbackandgainviaRayleighscattering(RS)andstimulatedRamanscatteringinalongpassivefiber,hasfundamentalscientificchallengesinpulsingoperationforitsremarkablecavity-freefeature.Forthetimebeing,stablepulsedRFLutilizingapassivemethodhasnotbeenreported.Here,weproposeandexperimentallyrealizethepassivespatiotemporalgain-modulation-inducedstablepulsingoperationofcounterpumpedRFL.Thankstothegoodtemporalstabilityofanemployedpumpingamplifiedspontaneousemissionsourceandthesuperiorityofthispulsegenerationscheme,astableandregularpulsetraincanbeobtained.Furthermore,thepumphysteresisandbistabilityphenomenawiththegenerationofhigh-orderStokeslightispresented,andthedynamicsofpulsingoperationisdiscussedafterthetheoreticalinvestigationofthecounterpumpedRFL.ThisworkextendsourcomprehensionoftemporalpropertyofRFLandpavesaneffectivenovelavenuefortheexplorationofpulsedRFLwithstructuralsimplicity,lowcost,andstableoutput.

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简介：新结构的材料的增加的力量和新结构的跨度，为更大的苗条由美学要求伴随了，正在导致长跨度的结构的更多的应用。在这份报纸，有用性控制技术和它的设计理论被学习。首先，一篇小说调节了质量更潮湿(TMD)与可控制的僵硬被开发。第二，为为导致人的负担建模的方法被建议，包括站起来，走，跳并且跑，并且为长跨度的地板反应的一个分析方法基于一个有限元素模型被建议。第三，为就人的舒适而言与一个多重TMD(MTMD)系统安装的长跨度的地板的一个设计方法被介绍，主要基于存在的研究文学。最后，一个图案，分析和领域测试包括北京奥林匹克派克公民会议中心，长沙新火车站和Xian在中国用几座大规模大楼被进行北火车站。分析并且MTMD系统用建议方法设计了的领域测试结果表演能够有效地减轻长跨度的地板结构的垂直颤动。在这份报纸介绍的学习为这些结构为类似的长跨度的地板的颤动有用性和控制系统的设计的分析提供重要引用。

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简介：肿瘤坏死因素(TNF)联系了导致apoptosisligand(TRAIL/APO2L)是在血缘的死亡受体的约会之上导致apoptosis的TNF基因总科的一个成员。当小道对正常房间相对无毒时，它有选择地在许多转变房间导致apoptosis。不过，胸肿瘤房间对小道的效果特别地抵抗。这里，我们报导在有cyclin依赖的kinase禁止者roscovitine的联合，落后于的暴露在检验的小道抵抗的乳癌房间线的多数导致了显著apoptosis。Roscovitine便于小道导致死亡的发信号的复杂形成和caspase-8的激活。FLICE禁止的蛋白质是的cFLIP(L)和cFLIP显著地下面调整的列在后面暴露到roscovitine并且，确实由siRNA的cFLIPisoforms击倒敏化的胸肿瘤房间到导致小道的apoptosis。另外，我们证明roscovitine强烈在胸肿瘤房间压制了Mcl-1表示和起来调整的E2F1蛋白质层次。显著地，由siRNA的Mcl-1的silencing敏化胸肿瘤房间到导致小道的apoptosis。而且，由减少的siRNA的E2F1蛋白质击倒在导致小道的apoptosis的roscovitine的敏化的效果。在摘要，我们的结果为roscovitine的pro-apoptotic影响揭示pleitropic机制，加亮它象在在有小道的联合的乳癌的一个反肿瘤代理人的潜力。

简介：Opticalwhisperinggallerymicrocavitieswithhigh-qualityfactorshaveshowngreatpotentialtowardachieveingultrahigh-sensitivitysensinguptoasinglemoleculeornanoparticle,whichraisesahugedemandonadeeptheoreticalinsightintothecrucialphenomenasuchasthemodeshift,modesplitting,andmodebroadeninginsensingexperiments.Hereweproposeanintuitivemodeltoanalyzethesephenomenafromtheviewpointofthenanoparticle-inducedmultiplescatteringoftheazimuthallypropagatingmode(APM).ThemodelunveilsexplicitrelationsbetweenthesephenomenaandthephasechangeandenergylossoftheAPMwhenscatteredatthenanoparticle;themodelalsoexplainstheobservedpolarization-dependentpreservationofoneresonanceandtheparticle-dependentredshiftorblueshift.Themodelindicatesthattheparticle-inducedcouplingbetweenthepairofunperturbeddegeneratewhisperinggallerymodes(WGMs)andthecouplingbetweentheWGMsandthefree-spaceradiationmodes,whicharewidelyadoptedincurrenttheoreticalformalisms,arerealizedviathereflectionandscattering-inducedfree-spaceradiationoftheAPM,respectively,andadditionallyexhibitsthecontributionofcrosscouplingbetweentheunperturbedWGMsandotherdifferentWGMstoformingthesplittingresonantmodes,especiallyforlargeparticles.

简介：Gradeassessmentofsteelisgenerallyperformedviathemetallographicmethod,whichistime-consumingandisnotabletoprovidetheelementaldistributioninformation.Inthispaper,wepresentamethodtomeasuretheglobularoxideinclusionratingsinsteelusinglaser-inducedbreakdownspec-troscopy（LIBS）.Themeasurementisperformedintwobasicsteps：steelsamplesarepolishedusingmetallographicsandpaperandtheA1203inclusionnumberandsizedistributioninamarkedareaareobservedusingscanningelectronmicroscope/energydispersiveX-rayspectroscopy（SEM/EDS）forfurtherLIBSscanninganalysis.ThethresholdintensitythatdistinguishessolublealuminumandinsolublealuminuminclusionsisdeterminedusingLIBScombinedwiththeSEM/EDSstatisticaldata.Carbonsteel（thesamplenumberisS9256）andbearingsteel（thesamplenumberisGCr15）areana-lyzedinscanningmode,andthenumberofA1203inclusionsindifferentsizerangesisobtainedfromthestatisticalinformationderivedfromtheA1203sizecalibrationcurve.Accordingtoheavyandthinseriesforglobularoxideinclusionsgradeassessment,themethodweproposeiscomparabletothetraditionalmetallographicmethodintermsofaccuracy;however,theprocessissimplifiedandthemeasurementspeedissignificantlyimproved.

简介：人的导致的pluripotent茎(iPS)房间类似于胚胎的茎(ES)房间，和罐头强烈地增殖并且区分进许多房间类型。然而，人的iPS房间的肝的区别还没被报导了。在这份报告，人的iPS房间被导致由一个逐步的协议区分进肝的房间。肝房间标记的表达式和人的iPS的肝相关的函数导出房间的房间被监视并且与区分的人的ES房间和主要人的hepatocytes的相比。在白天7点的约60%区分的人的iPS房间表示了肝的标记alphafetoprotein和白长袍的。在白天21点的区分的房间包括白朊Asecretion，肝糖合成，脲生产和可诱导的细胞色素P450活动展出了肝房间功能。肝的标记的表示和iPS的肝相关的功能导出房间的肝的房间比得上人的ES导出房间的肝的房间的。这些结果证明人的iPS房间，类似于人的ES房间，能高效地被导致区分房间进象hepatocyte一样。

简介：ANonlinearFluidDamping(NFD)intheformofthesquare-velocityisappliedintheresponseanalysisofVortex-InducedVibrations(VIV).Itsnonlinearhydrodynamiceffectsonthecoupledwakeandstructureoscillatorsareinvestigated.AcomparisonbetweenthecoupledsystemswiththelinearandnonlinearfluiddampingsandexperimentsshowsthattheNFDmodelcanwelldescriberesponsecharacteristics,suchastheamplificationofbodydisplacementatlock-inandfrequencylock-in,bothathighandlowmassratios.Particularly,thepredictedpeakamplitudeofthebodyintheGriffinplotisingoodagreementwithexperimentaldataandempiricalequation,indicatingthesignificanteffectoftheNFDonthestructuremotion.

简介：从铵孤立的RNA--并且对待硝酸盐的米饭叶子被用来屏蔽差别通过mRNA的表示基因微分显示。72个乐队的一个总数显得重要他们的差别和一些被反向的北、北的污点进一步证实。结果显示出那二基因，A-02(Oryzasativa干旱应力联系了mRNA)并且A-03(Zeamays为TFIIB相关的蛋白质的部分mRNA)在喂铵的米饭叶子是高度起来调整的。酶试金证明二抗氧化的酶，过氧化氢酶和peroxidase的活动，和一种non-enzymic抗氧化剂的内容，谷胱甘肽，比在喂硝酸盐的那些在喂铵的米饭叶子是显著地更高的，显示铵营养可能为米饭是有益的种在生长和开发期间改进压力抵抗。

简介：雄激素缺乏强烈与可勃起的机能障碍(编辑)被联系。不适当的阴茎动脉的血流动是到阴茎海绵体的血流动主要是的编辑的原因从内部pudendal动脉(国际语音学协会)导出的专业之一；然而，没有学习在国际语音学协会功能上评估了雄激素剥夺的效果。我们假设了那阉割损害国际语音学协会反应和结构，在我们的学习贡献编辑，Wistar雄的老鼠，8-week-old，被阉割并且在orchiectomy以后学习了30天。老鼠国际语音学协会的功能、结构的性质是坚定的分别地使用电线和压力肌动描记器系统。蛋白质表示被西方的污点和immunohistochemistry决定。血浆睾丸激素层次用IMMULITE1000免疫分析系统被决定。展出的阉割的老鼠损害了可勃起的功能，由减少的intracavernosal压力/平均数代表了动脉的压力比率。从阉割的老鼠的国际语音学协会展出了减少的phenylephrine-，电场刺激(EFS)导致了收缩并且减少导致醋胆素、导致EFS的vasodilatation。从阉割的老鼠的国际语音学协会展出了减少的内部直径，外部直径，动脉的墙的厚度，和代表性的区域。阉割减少了nNOS和肌动朊表示和增加的骨胶原表示，p38(Thr180/Tyr182)phosphorylation，以及caspase3劈开。在结论，雄激素缺乏与国际语音学协会反应和结构的缺陷被联系并且增加了表明标记的apoptosis。我们的调查结果建议导致缺乏的脉管的机能障碍是的那雄激素包含hypotrophic的一个事件国际语音学协会的脉管的改变。

简介：Lagrangian-Eulerianformulationsbasedonageneralizedvariationalprincipleoffluid-solidcouplingdynamicsareestablishedtodescribeflow-inducedvibrationofastructureundersmalldeformationinanincompressibleviscousfluidflow.Thespatialdiscretizationoftheformulationsisbasedonthemulti-linearinterpolatingfunctionsbyusingthefiniteelementmethodforboththefluidandsolidstructures.Thegeneralizedtrapezoidalruleisusedtoobtainapparentlynon-symmetriclinearequationsinanincrementalformforthevariablesoftheflowandvibration.Thenonlinearconvectivetermandtimefactorsarecontainedinthenon-symmetriccoefficientmatrixoftheequations.Thegeneralizedminimumresidual(GMRES)methodisusedtosolvetheincrementalequations.AnewstablealgorithmofGMRES-Hughes-Newmarkisdevelopedtodealwiththeflow-inducedvibrationwithdynamicalfluid-structureinteractionincomplexgeometries.Goodagreementbetweenthesimulationsandlaboratorymeasurementsofthepressureandbladevibrationaccelerationsinahydroturbinepassagewasobtained,indicatingthattheGMRES-Hughes-Newmarkalgorithmpresentedinthispaperissuitablefordealingwiththeflow-inducedvibrationofstructuresundersmalldeformation.