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简介：BACKGROUND:Transplantedmononuclearcell(MNC)ofumbilicalbloodcansurviveincentralnervoussystem(CNS)ofhostthroughbloodbrainbarrier,differentiateintonervercells,migratetodamagedsiteandintegratemorphologicalstrucghandfunctionwithnervecellsofhostsoastoimprovedeficienciesofsensatoryfunction,motorfunctionandcognitivefunctionandinfluenceonstrokesequela.OBJECTIVE:Toobservetheveintransplantationofhumanumbilicalcordbloodstemcells(HUCBSC)forimprovingneurologicalfunction,limbfuntionandactivityofdailylivingofpatientswithstrokeandevaluatethereliability.DESIGN:Self-controlledstudy.SETTING:DepartmentofNeurosurgery,theSecondPeople'sHospitalofZhengzhouCity;Red-crossedBloodCenterofHenanProvince;DepartmentofNeurosurgery,theFistAffiliatedHospitalofZhenzhouUniversity.PARTICIPANTS:Atotalof10patientswithstokesequelawereselectedfromDepartmentofCerebralSurgery,theSecondPeople'sHospitalofZhengzhouCityfromApriltoDecember2005.Therewere9malesand1femaleagedfrom35to75yearswiththemeanageof56years.AllofthemwerediagnosedwithCTandMRIexaminationandcoincidencewithdiagnosticcriteriaofstrokeestablishedbytheFourthNationalAcademicMeetingforCerebrovascularDisease.Allpatientsprovidedinformedconsent.METHODS:80-140mLumbilicalbloodoftermbirthofnewbornwasselectedhermeticallyandmaintainedinsterileplasticbag.Andthen,thebloodwascentrifugatedatthespeedof1500r/minfor30minutesat22℃inordertoseparateMNC,i.e.,HUCBSC.Inaddition,afterfinaldiagnosisduringhospitalization,strokepatientswereperfusedwithHUCBSCthroughsuperficialveinofbackofthehand.Eachpatientwasaveragelypenfusedwith6portionsofHUCBSC(cellularnumbers≥1×108/portion)andtheintervalbetweeneachportionwas1-7dayswiththemeanintervalof4days.MAINOUTCOMEMEASURES:①Neurologicalfunctionofstrokepatientswasevaluatedwithneurologicalfunctiondeficiency(NFD)befor

简介：客观：为了探索可行性构造遗传工程人，神经干细胞(hNSCs)由lentivirus调停了多表示基因以便为针的绳索损害(SCI)的进一步的研究提供接枝来源。方法：从人的流产胎的大脑外皮的人的神经干细胞被孤立并且有教养，然后，基因被lentivirus修改两个都表示绿荧光蛋白质(GFP)和老鼠neurotrophin-3(NT-3)；转基因的表示被荧光显微镜，胎儿的老鼠的背面的根中心和槽污点的方法检测。结果：遗传工程hNSCs成功地被构造。所有在荧光显微镜下面表示了明亮绿的荧光的遗传工程hNSCs被观察。转基因的hNSCs的调节媒介能导致从背面的根中心(DRG)挥舞长出的神经突。遗传工程hNSCs表示了高级NT-3which能被使用槽污点检测。结论：遗传工程hNSCs调停了bylentivirus能被构造多成功地表示基因。

简介：背景：到infarcted心肌层的间充质的干细胞(MSC)的全身的交货是这个策略遗体的吸引人的noninvasive策略，而是治疗学的效果高度争论。方法：心肌的梗塞被左前面的下降冠的动脉的短暂结扎为60min在女Sprague-Dawley老鼠导致。任何一个2.5????¤

简介：客观：为了由adipose-derivedstromal房间(ASC)在vitro导致adipocyte区别，与绿荧光灯的蛋白质(GFP)从转基因的老鼠收获了并且估计经由ASC的附件构造脂肪质的纸巾打字的可能性我骨胶原scaffolds.Methods：从GFP转基因的老鼠的腹股沟的胖垫被酶为ASC(主要文化)的隔离消化。在到ASC的三个段落的扩大以后，房间被ASC在vitro在为二个星期，和adipocyte区别中等的anadipogenic孵化被词法观察和油红估计染色的O。然后，他们被纳入为12个小时andco有教养的骨胶原脚手架，为2months由皮下注射的培植列在后面到裸体老鼠的背面的皮肤。最新形成的纸巾被检测由他染色。结果：有教养的主要干细胞是像成纤维细胞、显示出的活跃增长。在在anadipocyte区别媒介被孵化以后，在逐渐地并且最后积累的细胞质的类脂化合物微滴发展成成熟adipocytes，它出现了在染色的油红O积极。0。5厘米的~3new组织块在裸体老鼠的背面的皮下面被发现，它被荧光灯的观察作为成熟脂肪质的纸巾证实并且他染色。结论：ASC能成功地区分脂肪质的纸巾进成熟adipocytes，它作为intracytoplasmic类脂化合物微滴展出象adipocyte一样形态学和快车。它是与ASC和类型设计的脂肪质的纸巾的一个有效模特儿我骨胶原脚手架。

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简介：AbstractObjective:To elucidate the epidermal melanocyte distribution according to sex, age, and body part with the goal of providing benchmark data for the diagnosis and therapeutic effect evaluation of pigmentary skin diseases.Methods:Epidermal melanocytes and keratinocytes were assessed using direct immunofluorescence staining, and the melanocyte density and epidermal thickness were calculated. The obtained data were statistically analyzed using SPSS Version 20.0 software. An independent-samples t test was used to compare the data between two groups, while data among three or more groups were compared by one-way analysis of variance. Data correlations were evaluated using Pearson correlation analysis.Results:Melanocytes were uniformly distributed among the keratinocytes in the basal layer, and the average ratio of melanocytes to keratinocytes was 1:7. Among them, the ratio in males was 1:6.5 and that in females was 1:7.4, with no significant difference (P= 0.127). The melanocyte density gradually declined as age increased; the ratio was 1:5.8 before 50 years of age without an obvious downtrend. The average melanocyte density was 1:7.9 within 51 to 65 years of age and 1:8.5 at >65 years of age, and the difference was statistically significant (P < 0.01). Obvious differences were found in the melanocyte density among different body parts; in descending order, these densities were as follows: face (1:4.0) >neck (1:5.1) >hip (1:5.7) >upper limb (1:7.4) >lower limb (1:8.3) >lower back (1:9.2) >thorax and abdomen (1:9.9). The melanocyte density was not related to the epidermal thickness.Conclusion:The melanocyte density showed a declining trend with age and significantly changed after 50 years of age. The melanocyte density was associated with body part; specifically, the density in the face, neck, and hip was higher than that in the limbs and torso. However, the melanocyte density was not associated with sex or epidermal thickness.

简介：Similarlytootheradulttissues,ahierarchicalstructurehasbeenestablishedforthebrain,wherethedifferentiatedcelltypes(neurons,oligodendrocytesandastrocytes)aregeneratedfromprimaryprogenitorcells,knownastypeB

简介：有干细胞特征的前列腺癌症房间被他们在非支持者文化从单个房间形成自我更新的prostaspheres的能力在人的前列腺癌症房间线识别。Prostaspheres展出了增长，区别和茎的异构的表示联系房间的制造者CD44，ABCG2和CD133。有WNT禁止者的治疗减少了prostasphere尺寸和自强。相反，引起的Wnt3a的增加增加了prostasphere尺寸和自强，它与原子尾-catenin的重要增加被联系，角质素18，CD133和CD44表示。作为LNCaP和C4-2B癌症房间快车雄激素受体的一个高比例，我们决定了雄激素受体对手bicalutamide的效果。雄激素受体抑制减少了prostasphere尺寸和PSA的表示，但是没禁止prostasphere形成。这些效果与有干细胞特征和放大房间的运输的雄激素依赖者增长的房间的雄激素无关的自强一致。作为发信号的受动器尾-catenin能也与雄激素受体联系的正规WNT，我们为包含在WNT和雄激素受体活动之间的平衡的瘤繁殖建议一个模型。那将与干细胞特征和开车运输放大房间增长和区别影响一个癌症房间的自强。在结论，我们提供那项WNT活动独立于雄激素受体活动与干细胞特征调整前列腺癌症房间的自强的证据。WNT发信号的抑制因此有潜力与干细胞特征减少前列腺癌症房间的自强并且改进治疗学的结果。

简介：骨头导出髓的间充质的干细胞(MSC)是为房间移植在临床的应用程序显示出一个重要潜力的pluripotent干细胞。在现在的论文，proteomic技术被用来接近与猪的骨头髓MSC联系的蛋白质侧面并且在5-azacytidine(5-aza)的效果上调查MSC蛋白质的规定。超过1,700蛋白质种类根据胶化分析与MSC被分开。与控制MSC介绍的表达式相比，有起来调整的11个蛋白质点并且26在5-aza-treated房间的蛋白质模式下面调整。21蛋白质的一个总数被MALDI-TOF-MS分析成功地识别，在哪个之中一些有趣的蛋白质，例如高山哈B-crystallin，在A2的附属建筑，和stathmin1，被报导了通过不同发信号的小径在房间增长和区别包含。我们的数据应该为MSC区别和apoptosis的未来学习是有用的。

简介：瞄准：调查角色和骨头髓的潜在的机制在严重尖锐腹膜炎(树液)的间充质的干细胞(MSC)。方法：从SpragueDawley老鼠的胰腺的acinar房间随机被划分成三个组：非钠deoxycholate(SDOC)组(non-SODC组)，SDOC组，和MSC干预组织(即，MSC的一个合作文化系统和胰腺的acinar房间+SDOC)。房间幸存率，malonaldehyde(MDA)的集中，superoxidedismutase(草皮)的密度，浆液淀粉酶(AMS)分泌物评价并且喂奶脱氢酶(LDH)漏率在各种各样的时间点被检测。在分开的研究，SpragueDawley老鼠随机被划分成一个树液组或MSC组织的树液+。浆液AMS，MDA和草皮，interleukin(IL)-6,IL-10，和肿瘤坏死因素(TNF)-层次，肠的mucosa损害分数和小肠的mucosa的增殖的房间在注射任何一个MSC以后在各种各样的时间点被测量或进老鼠盐。在两学习，MSC的保护的效果被评估。结果：在vitro，胰腺的acinar房间和草皮的密度的房间幸存率显著地被减少，并且MDA，AMS分泌物率和LDH漏率的集中显著地与MSC干预组和Non-SDOC组相比在SDOC组被增加在每次指。在vivo，在SAP+MSC组的浆液AMS，IL-6，TNF-和疯水平比SAP组低；然而，浆液IL-10水平比SAP组高。浆液草皮水平比SAP组在高每次指，而在草皮水平的重要在组之间差别仅仅在24h以后被注意。肠的mucosa损害分数显著地被减少，小肠的mucosa的增殖的房间在注射MSC以后变得明显。结论：MSC能有效地减轻损害到胰腺的acinar房间和小肠的上皮，支持伤寒上皮的增长并且mucosa修理，与树液一起在老鼠稀释全身的发炎。

简介：Poly(lactide-co-glycolide)-poly(ethylene乙二醇)-poly(lactide-co-glycolide)(PLGA-PEG-PLGA)triblock共聚物是作为macroinitiator和亚锡的octoate与木钉通过LA和GA的戒指洞聚合被综合催化剂。进在水的答案的微粒自我装配的amphiphilic共聚物，和形成的hydrogels作为在相对高的集中的温度的增加(>15wt%)。hydrogel的有利degradability被证实由在vitro并且在vivo降级实验。好细胞并且thermogel的tissular相容性被表明。骨头髓的优秀粘附和增长间充质的干细胞为软骨织物工程赋予了PLGA-PEG-PLGAthermogellinghydrogel以迷人的前景。

简介：探索骨头的效果的目的有带hepatocyte生长因素的adenoviral向量的导出髓的间充质的干细胞(BMSC)transfected(HGF，Ad-HGF)在灼伤创伤愈合上。从男Wistar老鼠的方法BMSC用由密度坡度centrifugation中等、与包含20%胎儿的牛的浆液(FBS)的DMEM有教养的Percoll分开被分开并且净化。当时，BMSC是有在感染(MOI)的100复合的最佳的基因transduction效率的Ad-HGF的transfected。transfection和在暂停的HGF的表示的效率被流动cytometry检测，酶分别地连接了immunosorbent试金(ELISA)。32只雌老鼠受到90慥?灳瑩吗？

简介：Theextracellularmatrix,whichincludescollagens,laminin,orfibronectin,playsanimportantroleinperipheralnerveregeneration.Recently,aSchwanncell-derivedextracellularmatrixwithclassicalbiomaterialwasusedtomimictheneuralniche.However,extensiveclinicaluseofSchwanncellsremainslimitedbecauseofthelimitedorigin,lossofanautologousnerve,andextendedinvitroculturetimes.Inthepresentstudy,humanumbilicalcord-derivedmesenchymalstemcells(hUCMSCs),whichareeasilyaccessibleandmoreproliferativethanSchwanncells,wereusedtoprepareanextracellularmatrix.WeidentifiedthemorphologyandfunctionofhUCMSCsandinvestigatedtheireffectonperipheralnerveregeneration.Comparedwithanon-coateddishtissueculture,thehUCMSC-derivedextracellularmatrixenhancedSchwanncellproliferation,upregulatedgeneandproteinexpressionlevelsofbrain-derivedneurotrophicfactor,glialcell-derivedneurotrophicfactor,andvascularendothelialgrowthfactorinSchwanncells,andenhancedneuriteoutgrowthfromdorsalrootganglionneurons.ThesefindingssuggestthatthehUCMSC-derivedextracellularmatrixpromotesperipheralnerverepairandcanbeusedasabasisfortherationaldesignofengineeredneuralniches.

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简介：Humanplacenta-derivedmononuclearcells(MNC)wereisolatedbyaPercolldensitygradientandculturedinmesenchymalstemcell(MSC)maintenancemedium.Thehomogenouslayerofadherentcellsexhibitedatypicalfibroblastlikemorphology,alargeexpansivepotential,andcellcyclecharacteristicsincludingasubsetofquiescentcells.Invitrodifferentiationassaysshowedthetripotentialdifferentiationcapacityofthesecellstowardadipogenic,osteogenicandchondrogeniclineages.FlowcytometryanalysesandimmunocytochemistrystainshowedthatplacentalMSCwasahomogeneouscellpopulationdevoidofhematopoieticcells,whichuniformlyexpressedCD29,CD44,CD73,CD105,CD166,laminin,fibronectinandvimentinwhilebeingnegativeforexpressionofCD31,CD34,CD45andα-smoothmuscleactin.Mostimportantly,immuno-phenotypicanalysesdemonstratedthatthesecellsexpressedclassImajorhistocompatibilitycomplex(MHC-Ⅰ),buttheydidnotexpressMHC-Ⅱmolecules.Additionallythesecellscouldsuppressumbilicalcordblood(UCB)lymphocytesproliferationinducedbycellularornonspecificmitogenicstimuli.Thisstronglyimpliesthattheymayhavepotentialapplicationinallografttransplantation.SinceplacentaandUCBarehomogeneous,theMSCderivedfromhumanplacentacanbetransplantedcombinedwithhematopoieticstemcells(HSC)fromUCBtoreducethepotentialgraft-versus-hostdisease(GVHD)inrecipients.

简介：雄激素缺乏是影响成年人的物理混乱，但是能也使受危险不仅孩子健康。因为到使用传统的雄激素代替治疗有许多劣势，我们此处试图探索人的脐的绳索的使用为雄激素缺乏的治疗的间充质的干细胞。我们移植了进乙烷dimethanesulfonate(版本)的睾丸的间充质的干细胞导致了的CM-Dil-labeled人的脐的绳索雄的老鼠性腺机能减退模型。在移植以后的21天，我们发现在治疗组的血睾丸激素层次比控制组的高(P=0.037)，并且用immunohistochemistry和流动cytometry，我们观察到一些CM-Dil-labeled房间为细胞色素P450表示了Leydig房间标记，家庭11，亚科A，多肽1，并且3--hydroxysteroid脱氢酶。我们然后恢复了这些房间并且观察到他们仍然能在vitro增殖。现在的学习证明从人的脐的绳索的间充质的干细胞可以为男性腺机能减退病人的治疗组成一种有希望的治疗学的形式。

简介：POU抄写因素OCT4不仅在维持pluripotent和房间而且幕作为通过基因剂量的一个房间命运决定因素完成的胚胎的茎(ES)的自我更新的状态起一个必要作用。然而，控制细胞内部的OCT4蛋白质水平的分子的机制留下逃犯。这里，我们报导那人的WWP2，E3ubiquitin(Ub)蛋白质ligase，通过它的WW领域明确地与OCT4交往并且在vitro并且在vivo提高OCT4的Ub修正。我们首先证明在人的ES房间的内长的OCT4能被Ubpost-translationally修改。而且，我们发现WWP2以一种剂量依赖者方式，和WWP2的活跃地点半胱氨酸残余通过26Sproteasome支持了OCT4的降级在OCT4上为它的酶的活动和解朊的效果被要求。显著地，我们当WWP2表示是由特定的RNA干扰(RNAi)的downregulated时，内长的OCT4蛋白质水平显著地被提高的数据表演，建议那WWP2是为在人的ES房间维持合适的OCT4蛋白质水平的一个重要管理者。而且，北污点分析证明WWP2抄本在多样的人的织物/器官是广泛地在场的并且高度在无差别的人的ES房间表示了。然而，它的表示水平快速在区分的人的ES房间以后被减少，显示WWP2表示力量发展地被调整。我们的调查结果证明WWP2是在人的ES房间的OCT4蛋白质水平的一个重要管理者。