简介：CellResearchispublishedmonthlybyNaturePublishingGroupinpartnershipwithShanghaiInstitutesforBiologicalSciences(SIBS),ChineseAcademyofSciences(CAS).CellResearch,whichpublishesexclusivelyinEnglish,isChina'sleadingjournalinthelifescienceswithacurrentImpactFactorof3.426(Thomson-ISI,PA,USA2007).Thejournalisindexed/abstractedinIndexMedicus/Medline/PubMed,ScienceCitationlndex,CurrentContents-lifeSciences,SciSearchandISIWebofScience,ChemistryAbstracts,BIOSIS,VINITI,Cbinainfo(CD&Web),CJFD(CD&Web),CJCDandCEPS,etc.ScopeCellResearchisaninternationaljournalpublishingpeer-reviewedoriginalarticles,invitedreviews,commentaries,andshortcommunications.Thejournalhasaninternationalauthorshipandabroadscopeinbasicresearchofcellbiology,molecularcellbiology,includingcellgrowthanddifferentia-tion,signaltransduction,apoptosis,stemcells,development,immunology,neurosciences,plantcellbiology,chromatinmodulation,epigeneticsandtranscription.EditorialManuscriptsshouldbesubmittedonlineathttp://www.nature.com/crorhttp://www.cell-research.com.Howeverifyouhaveproblems,pleasecontactEditorialOfficeatcellres@sibs.ac.cn.DetailedInstructionsforAuthorsarealsoavailableattheabovewebsites.PublisherExceptforcompaniesbasedinmainlandChina,allbusinesscorrespondenceandenquiriesaboutsupplementpublication,sponsorshipopportunitiesandadvertisingshouldbeaddressedtoNaturePublishingGroup(NPG).NickCampbell,AssociatePublisher,ExecutiveEditor:NPGNatureAsia-Pacific,MacmillanPublishers,LockedBag1,PrahranVIC3181,Australia.Tel:+61(0)398251028;Fax:+61(0)398251010.E-mail:n.campbell@natureasia.com

简介：

简介：

简介：

简介：GelatinaseA(MMP-2)isconsideredtoplayacriticalroleincellmigrationandinvasion.Theproteinaseiscercetedfromthecellasaninactivezymogen.InvivoitispostulatedthatactivationofprogelationaseA(proMMP-2)takesplaceonthecellsurfacemediatedbymembrane-typematrixmetalloproteinases(MT-MMPs).RecentstudieshavedemonstratedthatproMMP-2isrecruitedtothecellsurfacebyinteractingwithtissueinhibitorofmetalloproteinases-2(TIMP-2)boundtoMT1-MMPbyformingaternarycomplex.FreeMT1-MMPcloselylocatedtotheternarycomplexthenactivatesproMMP-2onthecellsurface.MT1-MMPisfoundinculturedinvasivecancercellsattheinvadopodia.TheMT-MMP/TIMP-2/MMP-2systemthusprovideslocalizedexpressionofproteolysisoftheextracellularmatrixrequiredforcellmigration.

简介：pluri有势力干细胞的能力为治疗长期、退化的疾病在新奇房间代替治疗学的开发保持大诺言修理干细胞在住的纸巾。然而，众多的报告证明甚至在一个自体同源的背景，那干细胞治疗触发淋巴细胞渗入和发炎。因此，要回答的一个重要问题是主人免疫系统怎么对嫁接自体同源的干细胞或allogeneous干细胞作出回应。在这简短评论，我们在这块地里在几个相关区域总结进步，包括一些我们的数据，在四节：(1)干细胞的immunogenicity；(2)禁止有免疫力的拒绝到allograft干细胞的策略；(3)对癌症干细胞的有免疫力的回答；并且(4)在有免疫力的规定的间充质的干细胞。我们这些和有免疫力的系统茎细胞相互影响的另外的方面上的理解的改进将极大地便于茎的发展为再生目的基于房间的治疗学。

简介：AbstractBackground:Acute kidney injury (AKI) is a common and serious complication following lung transplantation (LTx), and it is associated with high mortality and morbidity. This study assessed the incidence of AKI after LTx and analyzed the associated perioperative factors and clinical outcomes.Methods:This retrospective study included all adult LTx recipients at the China-Japan Friendship Hospital in Beijing between March 2017 and December 2019. The outcomes were AKI incidence, risk factors, mortality, and kidney recovery. Multivariate analysis was performed to identify independent risk factors. Survival analysis was presented using the Kaplan-Meier curves.Results:AKI occurred in 137 of the 191 patients (71.7%), with transient AKI in 43 (22.5%) and persistent AKI in 94 (49.2%). AKI stage 1 occurred in 27/191 (14.1%), stage 2 in 46/191 (24.1%), and stage 3 in 64/191 (33.5%) of the AKI patients. Renal replacement therapy (RRT) was administered to 35/191 (18.3%) of the patients. Male sex, older age, mechanical ventilation (MV), severe hypotension, septic shock, multiple organ dysfunction (MODS), prolonged extracorporeal membrane oxygenation (ECMO), reintubation, and nephrotoxic agents were associated with AKI (P < 0.050). Persistent AKI was independently associated with preoperative pulmonary hypertension, severe hypotension, post-operative MODS, and nephrotoxic agents. Severe hypotension, septic shock, MODS, reintubation, prolonged MV, and ECMO during or after LTx were related to severe AKI (stage 3) (P < 0.050). Patients with persistent and severe AKI had a significantly longer duration of MV, longer duration in the intensive care unit (ICU), worse downstream kidney function, and reduced survival (P < 0.050).Conclusions:AKI is common after LTx, but the pathogenic mechanism of AKI is complicated, and prerenal causes are important. Persistent and severe AKI were associated with poor short- and long-term kidney function and reduced survival in LTx patients.

简介：Primarysclerosingcholangitis（PSC）isachronicprogressiveinflammatorydiseaseaffectingthebileducts,leadingtofibrosisandeventuallycirrhosisinmostpatients.Itsetiologyisunknownandsofarnoeffectivemedicaltherapyisavailable.Livertransplantation（LTX）istheonlycurativetreatmentandatpresentPSCisthemainindicationforLTXintheScandinaviancountries.ClosetohalfofthePSCpatientsexperienceoneormoreepisodesofacutecellularrejection（ACR）followingtransplantationandapproximately1/5ofthetransplantedpatientsdeveloprecurrentdiseaseinthegraft.Inaddition,somereportsindicatethatACRearlyafterLTXforPSCcaninfluencetheriskforrecurrentdisease.Fortheseimportantpost-transplantationentitiesaffectingPSCpatients,wehavereviewedthecurrentliteratureonepidemiology,pathogenesis,treatmentandthepossibleinfluenceofrejectionontheriskofrecurrentdiseaseintheallograft.

简介：AbstractIn January 2019, the fourth rabies case caused by organ transplantation was noticed in China, with the conditions of one per year for the recent four years. Different from the previous cases, there were no definite epidemiological histories of exposure or rabies-related symptoms from this patient. This case strongly supports the call for the legislation of establishing a national-level management that will incorporate the screening programs on donors prior to the practice of organ transplantation to reduce the risks on rabies caused by organ transplantation.

简介：

简介：无

简介：

简介：

简介：Withtheexceptionofmatureerythrocytes,cellswithinthehumanhematopoieticsystemarecharacterizedbythecellsurfaceexpressionofthepan-leukocytereceptorCD45.Here,weidentifyanovelsubsetamongmononuclearcordbloodcellsdepletedoflineagecommitmentmarkers(Lin-)thataredevoidofCD45expression.Surprisingly,functionalexaminationofLin-CD45-cellsalsolackingcellsurfaceCD34revealedtheywerecapableofmultipotentialhematopoieticprogenitorcapacity.Co-culturewithmouseembryoniclimbbudcellsdemonstratedthatLin-CD45-CD34-cellswerecapableofcontributingtocartilagenodulesanddifferentiatingintohumanchondrocytes.BMP-4,amesodermalfactorknowntopromotechondrogenesis,significantlyaugmentedLin-CD45-CD34-differentiationintochondrocytes.Moreover,unlikeCD34+humanhematopoieticstemcells,Lin-CD45-CD34-cellswereunabletoproliferateorsurviveinliquidcultures,whereassingleLin-CD45-CD34-cellswereabletochimerizetheinnercellmass(ICM)ofmurineblastocystsandproliferateinthisembryonicenvironment.OurstudyidentifiesanovelpopulationofLin-CD45-CD34-cellscapableofcommitmentintobothhematopoieticandchondrocyticlineages,suggestingthathumancordbloodmayprovideamoreubiquitoussourceoftissuewithbroaderdevelopmentalpotentialthanpreviouslyappreciated.

简介：(GFI1)生长因素独立1为成年造血的干细胞(HSC)的哺乳动物的淋巴细胞和neutrophils和维护的成熟是重要的。在胚胎的造血作用的GFI1的角色是很好描绘的更少。通过一幅enhancer陷井屏幕和生物信息学分析，我们识别了Gfi1(命名gfi1.1)的一个斑马鱼相当或相同的事物并且在胚胎发育期间分析了它的功能。内长的gfi1.1基因和在它的genomic地点附近插入的GFP记者基因的表示在斑马鱼胚胎的造血的房间被检测。Morpholino(瞬间)gfi1.1击倒scl，lmo2，c-myb，mpo，rag1，gata1和血红素高山的减少的表示哈embryonic-1(hbae1)，以及胚胎的血红素的全部的数量，而是pu.1和l-plastin的增加的表示。在一样的条件下面，瞬间注射没影响涉及的标记脉管并且专业版肾的开发。相反地，在经由信使rna的gfi1.1的表示上，注射提高了gata1的表示，但是禁止了pu.1的表示。这些调查结果建议Gfi1.1在调整胚胎的erythroid和myeloid系决心的平衡起一个关键作用，并且也在斑马鱼期间为淋巴细胞和granulocytes的区别被要求胚胎开始。

简介：AbstractCell-cell communication is the basis of physiological processes and cell signals. The disease occurs when the cells do not adequately communicate and the messages are blocked. With ligand-receptor interaction databases and single-cell RNA sequencing (scRNA-seq) databases, we can detect intercellular signaling and reconstruct the cell-cell communications among different cell types. This review summarized the computational approaches for analyzing the cell-cell communication based on scRNA-seq data and discussed its applications in carcinogenesis and COVID-19. We believe that this review will accelerate the scRNA-seq data deciphering and facilitate the cell-cell communication studies for complex physiological processes, such as carcinogenesis and SARS-CoV-2 infection.

简介：

简介：

简介：ＥＦＦＥＣＴＳＯＦＡＰＡＴＩＴＥＣＥＲＡＭＩＣＳＯＮＣＥＬＬＧＲＯＷＴＨＳＡＮＤＤＮＡＳＹＮＴＨＥＳＥＳＩＮＨＵＭＡＮＯＳＴＥＯＢＬＡＳＴＬＩＫＥＣＥＬＬＥＦＦＥＣＴＳＯＦＡＰＡＴＩＴＥＣＥＲＡＭＩＣＳＯＮＣＥＬＬＧＲＯＷＴＨＳＡＮＤＤＮＡＳＹＮＴＨ...

简介：Tostudytheroleofnaturalkiller(NK)cellsinTcellrecruitmentinmurineliverinfectedwithvirus,micewereintravenouslyinjecteddailywithanti-NK1.1^+antibodytodepleteNKcells.Lymphocytesinthelivertissueofmiceinfectedwithtype5adenovirusdepletedintheE1andE3regionswereassessedbyfluorometricactivatedcellsorting(FACS).Ex-pressionofchemokineIP-10anditsreceptorCXCR3mRNAintheliver,hepaticlymphocytesandspleentissuewereexaminedbyreversetranscriptionpolymerasechainreaction(RT-PCR).Serumalmfineaminotransferase(ALT)wasmeasuredasanindicatorofliverinjury.Itwasfoundthatinfectionofadenovimsandanfi-Fasmonoclonalantibody(mAb)intomicecausedliverinjuryandhighexpressionofinterfemn-γinducibleprotein-10(IP-10)mRNAintheliver.Anfi-NK1.1^+mAb,whichwasintraperitoneallyinjectedintothemiceinfectedwithadenovirus,suppressesTcellrecruitmentandexpressionofIP-10mRNAinthehver.Slighterhverinjurywasalsoobserved.Afterviresinfection,expressionofCXCR3mRNAinspleenandhvertissuewasobservedatdifferenttime.TheresultssuggestedthatTcellrecruitmentwasinitiatedbyNKcelldependentchemokineIP-10,whichinducedactivatedTcellspriminginthespleentothehverofthemouse.NKcellsplayedakeyroleinTcellrecruitmentintheliverofmouseinfectedwithadenovims.