简介:Anewdi-functionadsorbent(JN-3)waspreparedbysulfhydrylmodified.ComparingwithAmberliteXAD-4andNDA-150,theequilibriumadsorptionforphenolontheJN-3fromaqueoussolutionswastested,perfectadsorptioncapacitywasshown.Pb2+canbealsoremovedbyJN-3becauseofthechelateinteractionbetweensulfhydrylgroupsandmetalions.Thisadsorbentcouldbeusedinremovalofcombinepollutantssuchasphenoliccompoundsandheavymetalionsfromwastestreams.
简介:Usingfirst-principlescalculations,wesystematicallystudythedissociationsofO2moleculesondifferentultrathinPb(111)films.AccordingtoourpreviousworkrevealingthemolecularadsorptionprecursorstatesforO2,wefurtherexplorewhytherearetwonearlydegenerateadsorptionstatesonPb(111)ultrathinfilms,butnoprecursoradsorptionstatesexistingatallonMg(0001)andAl(111)surfaces.Thereasonisconcludedtobethedifferentsurfaceelectronicstructures.FortheO2dissociation,weconsiderboththereactionchannelsfromgas-likeandmolecularlyadsorbedO2molecules.WefindthattheenergybarrierforO2dissociationfromthemolecularadsorptionprecursorstatesisalwayssmallerthanthatfromO2gas.ThemostenergeticallyfavorabledissociationprocessisfoundtobethesameondifferentPb(111)films,andtheenergybarriersarefoundtobeinfluencedbythequantumsizeeffectsofPb(111)films.
简介:摘要目的探讨全反式维甲酸(ATRA)对骨肉瘤143B细胞增殖和侵袭的影响及其可能的调控机制。方法采用不同浓度ATRA处理人骨肉瘤143B细胞,选取影响细胞增殖的最佳浓度和处理时间。分别采用MTS法、Transwell迁移和侵袭实验检测ATRA处理后143B细胞增殖、迁移和侵袭能力变化。采用实时荧光定量聚合酶链反应(qRT-PCR)和蛋白质印迹法检测ATRA处理后骨肉瘤143B细胞中miRNA-34a(miR-34a)、E2F1和Eag1的表达变化。干扰miR-34a和过表达E2F1,检测143B细胞增殖、侵袭和迁移能力以及miR-34a、E2F1和Eag1表达的变化。结果ATRA浓度为10 μmol/L、处理72 h对143B细胞增殖的抑制作用最明显。10 μmol/L ATRA作用72 h的迁移细胞数、侵袭细胞数均低于阴性对照组[(73±3)个比(182±5)个,t=21.46,P<0.01;(94±3)个比(203±7)个,t=13.70,P<0.01]。10 μmol/L ATRA可促进143B细胞中miR-34a的表达,抑制Eag1和E2F1的表达(均P<0.01)。与ATRA组比较,处理72 h后ATRA+miR-34a干扰组可恢复细胞的增殖能力[细胞存活率(41.0±2.2)%比(25.0±3.6)%,t=108.68,P<0.01]。与ATRA组比较,ATRA+miR-34a干扰组可恢复细胞的迁移和侵袭能力[(122±14)个比(64±10)个,t=21.06,P<0.01;(103±10)个比(59±8)个,t=24.27,P<0.01],并可促进细胞中Eag1和E2F1的mRNA和蛋白表达(均P<0.01)。与ATRA组相比,ATRA+E2F1过表达组可恢复细胞的增殖能力[细胞存活率(40.0±3.4)%比(24.0±3.1)%,t=108.74,P<0.01],可恢复细胞迁移和侵袭能力[(78±12)个比(29±8)个,t=13.52,P<0.01;(75±12)个比(49±10)个,t=6.28,P<0.01)],并促进细胞中Eag1和E2F1的mRNA和蛋白表达(均P<0.01)。结论ATRA通过调控miRNA-34a-E2F1-Eag1信号通路抑制骨肉瘤细胞增殖和侵袭,其可能成为骨肉瘤有效的治疗药物之一。
简介:摘要:为探讨料姜石对水中重金属Pb的吸附效果,本文研究了不同料姜石用量、不同时间及不同温度下料姜石对重金属Pb的吸附效果。研究表明,料姜石用量越多,吸附效果越明显,当料姜石用量为10g时,吸附基本达到饱和状态;同时,吸附时间越长,吸附效率越高;然而,当温度越高时,吸附效率明显下降。
简介:TheproblemsofcharacteristicpolynomialassignmentinFomasini-Marchesini(F-M)modelⅡof2-Dsystemsareinvestigated.Thecorrespondingclosed-loopsystemsdescribedbyF-MmodelIIareobtainedviathestatefeedback.Usingthealgebraicgeometrymethod,thecharacteristicpolynomialassignmentinthedosed-loopsystemsisdiscussed.Intermsofthetheoryofalgebraicgeometry,theproblemofcharacteristicpolynomialassignmentistransferredtotheonewhetherarationalmappingisonto.SufficientconditionsforalmostarbitraryassignmentcoefficientsofcharacteristicpolynomialinF-MmodelⅡof2-Dsystemsviastatefeedbackarederived,andtheyareavailableformulti-inputcases.Italsohasbeenshownthatthismethodcanbeappliedtoassignthecharacteristicpolynomialwithoutputfeedback.Thesufficientconditionsforalmostarbitraryassignmentcoefficientsofcharacteristicpolynomialofmulti-input2-DsystemsdescribedbyF-MmodelⅡwithoutputfeedbackareestablished.
简介:Basedonloadseparationtheory,theloadseparationparameterSpbmethodisaneffectiveapproachforestimatingtheJ-resistancecurvefromrecordsofloadversusdisplacementdirectly,usingonesharpcrackedspecimenandanadditionalreferencebluntcrackedspecimen.However,theeffectofthereferencebluntcrackedspecimenonJ-resistancedeterminationwasnotexplicitlyconsideredinpastwork.Inthispaper,amodifiedloadseparationparameterSpbmethodwasdevelopedtoeliminatethiseffect,andthenauniqueestimationofinstantaneouscracklengthforonesharpcrackedspecimencouldbeobtained.Furthermore,aforcedbluntingcalibrationmethodwasalsoadoptedtodeterminetheinstantaneouscracklengthintheloadinseparableregion,referringtoanormalizationmethod.ExperimentsonsteamturbinerotatorsteelCr2Ni2MoVwerecarriedouttoestimateJ-resistancecurvesusinganunloadingcompliancemethod.Byremovingunloadandreloaddatafromload-displacementrecords,theJ-resistancecurveforthesamesharpcrackedspecimenwasestimatedusingthemodifiedseparationparameterSpbmethod.TheresultsindicatethatthemodifiedSpbmethodcompletelyeliminatestheeffectofthereferencebluntcrackedspecimenontheinstantaneouscracklengthdeterminationofthesharpcrackedspecimen.However,differentJ-resistancecurvesinasmallrangeofcrackextensionarepresentwhendifferentbluntingcoefficientsareusedinthebluntinglineequation.TheJ-resistancecurveobtainedfromthemodifiedSpbmethodagreeswellwiththatobtainedfromthecompliancemethod.