简介:Objective:ToinvestigatewhethertheBc1-2antisenseoligonucleotide(ASODN)mayenhanceradiation-inducedapoptosisinRajicellline.Methods:Cellsurvivingfractionwasdeterminedusingthetrypanbluedyeexclusionassay.Theexpressionlevelofbc1-2proteinwasassayedbyimmunofluorescenceusingfluoresceisothiocyanatelabel.ApoptosiswasdetectedbyGiemsastainingandflowcytomertriccellcycleanalysis.Results:ItwasfoundthatBc1-2ASODNcombinedwithradiationhadsignificantlyreducedthenumberofviablecells(P<0.05).TherewasnodifferenceoncellsurvivalbetweenmismatchBc1-2oligodeoxynucleotide/radiationcombinationandradiation-treatedcellsalone.Bc1-2ASODNcombinedwithradiationcouldsignificantlyinhibitexpressionofBc1-2proteininRajicells(P<0.05).CellstreatedwithBc1-2ASODNcombinedwithradiationat72hdisplayedclassicapoptoticchanges.ApoptosisratesofRajicellstreatedwithBc1-2oligodeoxynucleotide/radiationcombinationandradiation-treatedcellsalone,respectively.Conclusion:Bc1-2antisenseoligonucleotidecanenhanceradiation-inducedapoptosisinRajicellline.
简介:摘要目的在建立腹主动脉粥样硬化大鼠模型基础上,通过动物实验,探讨心脑通络液对动脉粥样硬化大鼠病变腹主动脉Bc1-2表达的影响,为治疗动脉粥样硬化的临床用药提供实验依据。方法选取健康雄性大鼠40只,动物随机分为5组,即空白组、模型组、阿托伐他汀组、心脑通络液低剂量组、心脑通络液高剂量组。参照文献,采用高脂饲料饲养结合球囊损伤腹主动脉内膜制备动脉粥样硬化模型,连续喂养4周并结合球囊损伤腹主动脉内膜后,继续给药10周,末次给药后24小时,动物处死,取材。观察心脑通络液对动脉粥样硬化大鼠病变腹主动脉Bc1-2表达的影响.结果1.腹主动脉粥样硬化大鼠模型复制成功;2.心脑通络液可增强动脉粥样硬化大鼠腹主动脉细胞Bc1-2表达;结论心脑通络液可增强动脉粥样硬化大鼠腹主动脉细胞Bc1-2表达,起到抗凋亡作用。
简介:<正>Financematters—notleastin(civil)war.Evenbeyondthefinancialmeasureinitselftofinancethewarthereisthepotentialitytoconstructaconceptofafriendlyleader,oranenemywhobreakswiththe(unwritten)rulesofpolitics.AsmallepisodeinCaesar’sBellumCivileclearlyshowshowtoplaywithwordstoundermineauthority—herePompeius’:
简介:贵刊2004年第3期在《问题·争鸣》栏里登载了戴、代二位老师的《“1:2”不能写成“1/2”吗》一文,认为1:2可以写成1/2。2005年第二期又刊了王老师的《也谈“1:2”与“1/2”》一文,他认为,比就应写成“1:2”的形式,而�
简介:目的构建shRNA并干扰肝癌HepG2细胞中BC047440基因,观察其对肝癌HepG2细胞增殖的影响。方法根据BC047440基因序列,设计和合成两条特异表达该基因shRNA序列并克隆载体pGenesil-1,转染肝癌HepG2细胞并用定量PCR观察抑制BC047440基因表达的效果,并通过MTr法和流式细胞仪检测细胞增殖及细胞周期的变化。结果酶切和序列测定提示成功构建了特异表达BC047440基因的shRNA1和shRNA2两个质粒。shRNA1和shRNA2对BC047440mRNA的抑制率分别为80.22%和58.63%。干扰BC047440基因后,肝癌HepG2细胞增殖受到明显抑制,主要停滞在s期。结论构建的shRNA能有效干扰肝癌HepG2细胞BC047440基因的表达,并抑制肝癌HepG2细胞的增殖,提示BC047440基因和肝癌HepG2细胞增殖有关。
简介:二次根式(a~2)~(1|2)和((a~2)~(1|2))有什么区别吗?主要表现在下面三个方面:1.读法不同.(a~2)~(1|2)读作根号a的平方,而((a~2)~(1|2))读作括号根号a括号的平方.2.表示的意义不同.(a~2)~(1|2)是求a~2。的算术平方根.((a~2)~(1|2))求的是a的算术平方根的平方.一个表示求一
简介:MakingsenseofMarkAntony’spoliticalandmilitarydealingsintheEasternMediterraneanintheperiodfromtheBattleofPhilippi(in42BC)~2untiltheBattleofActium(in31)isnotastraightforwardmatter.WithAntonyhavingbeenbroughtintodisreputeunderthehandofOctavian/Augustus,modernobserversareconstantlyfacedwiththedifficultyofestablishingtowhatextenttheliterary
简介:Theground-stateandthermodynamicpropertiesofquantummixed-spinchainsof1/2-1/2-1-1and3/2-3/2-1-1areinvestigatedbyaquantumMonteCarlosimulationwiththeloop-clusteralgorithm.For1/2-1/2-1-1chain,wefindithastwophasesseparatedbyanenergy-gapvanishingpointintheground-state.For3/2-3/2-1-1chain,thenumericalresultsshowtwoenergy-gapvanishingpointsisolatedbydifferentphasesinitsground-state.Ourcalculationsindicatethatallthesegroundstatephasescanbeunderstoodbymeansofvalence-bond-solidpicture,andthethermodynamicbehavioratfinitetemperaturesiscontinuousasafunctionofparameterα=J2/J1.
简介:ThesplittingofpotentialenergylevelsforgroundstateX2ΠgofOx2(x=+1,1)underspin–orbitcoupling(SOC)hasbeencalculatedbyusingthespin–orbit(SO)multi-configurationquasi-degenerateperturbationtheory(SO-MCQDPT).TheirMurrell–Sorbie(M–S)potentialfunctionsaregained,andthenthespectroscopicconstantsforelectronicstates2Π1/2and2Π3/2arederivedfromtheM–Sfunction.TheverticalexcitationenergiesforOx2(x=+1,1)areν[O+12(2Π3/2→X2Π1/2)]=195.652cm1,andν[O12(2Π1/2→X2Π3/2)]=182.568cm1,respectively.Allthespectroscopicdataforelectronicstates2Π1/2and2Π3/2aregivenforthefirsttime.