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简介:摘要HIV-1储存库的持续存在是治愈HIV的主要障碍,在临床研究中,需要可靠的生物标志物对其进行标记。HIV-1 DNA在HIV-1储存库中可被持续检测到,在HIV-1感染诊断、预测病毒反弹和监测治疗效果等方面具有重要应用价值。PCR的检测技术是临床上常用的HIV-1 DNA检测方法,随着技术的不断创新与进步,可更准确地通过定性或定量检测感染细胞中总的、整合的和未整合的HIV-1 DNA。感染细胞中不同形式的HIV-1 DNA作为生物标志物在HIV感染监测和艾滋病治疗相关研究中报道日益增多。本文对感染细胞中HIV-1 DNA的检测方法及其作为生物标志物的临床应用进展进行综述。
简介:法医DNA分析技术在过去的20多年里取得了长足的进步,在法医物证鉴定中功不可没;而随着更多新技术的出现,这项技术也只有得到继续发展才能适应当今法医物证鉴定的新需要。
简介:Linkerswereassembledonaglasssurfacebasedonthehydrolysisandcondensationof3-glycidoxy-propyltrimethoxysilane(GPS).AftertheassemblyofGPS,fourapproachesweretriedtoopentheendingepoxidegroupofGPSortofurtherelongatethelinkers.TheeffectoftheseapproachesonDNAinsitusynthesisandhybridizationwasinvestigated.Forthespacingofthesynthesisinitiationsites,thewettabilityofthesupportandthelengthofthelinkinggroupthatattachestheinitiationsitetothesurfacehavedirectinfluencesontheyieldofcouplingreactionsandthesubsequenthybridizationevents.X-rayphotoelectronspectroscopy(XPS)andmeancontactanglesofdeionizedwateroftheaboveslidesweremeasuredtoassessthelinker'scharacteristicsineachprocedure.Itwasprovedthattheglassslidesweresuccessfullymodifiedandbecameexcellentsupportsfortheoligonucleotidessynthesis.Inaddition,itprovedbestfortheinsituoligonueleotidessynthesisthataglassslidewasinturntreatedwithethylenediamine,glutaradehyde,ethanolamineandsodiumborohydridesolutionatambienttemperatureaftersilanizedwithGPS.
简介:Inthisstudy,theentiremitochondrialDNA(mtDNA)controlregion(CR)ofPholisfangiwasamplifiedviapolymerasechainreactionfollowedbydirectsequencing.ThelengthofthemtDNACRconsensussequenceofP.fangiwas853bpinlength.Inaccordancewiththerecognitionsitesaswerepreviouslyreportedinfishspecies,themtDNACRsequenceofP.fangicanbedividedinto3domains,i.e.,theextendedterminalassociatedsequence(ETAS),thecentralconservedsequenceblock(CSB),andtheCSBdomain.Inaddition,thefollowingstructureswereidentifiedinthemtDNACRsequenceofP.fangi:2ETASsintheETASdomain(TASandcTAS),6CSBsinthecentralCSBdomain(CSB-FtoCSB-A),and3CSBsintheCSBdomain(CSB-1toCSB-3).ThesedemonstratedthatthestructureofthemtDNACRofP.fangiwassubstantiallydifferentfromthoseofmostotherfishspecies.ThemtDNACRsequenceofP.fangicontainedoneconservedregionfrom656bpto815bp.Similartomostotherfishspecies,P.fangihasnotandemrepeatsequencesinitsmtDNACRsequence.PhylogeneticanalysisbasedonthecompletemtDNACRsequencesshowedthattherewerenogeneticdifferenceswithinP.fangipopulationsofthesamegeographicaloriginandbetweenP.fangipopulationsofdifferentgeographicalorigins.